青岛大学学报（医学版）2024，Vol.60Issue(2)：169-173,5.DOI:10.11712/jms.2096-5532.2024.60.058
百里醌对烟曲霉菌角膜炎的抗炎作用及其机制
Anti-inflammatory effects of thymoquinone against Aspergillus fumigatus keratitis and its mechanism of action
摘要
Abstract
Objective To investigate the anti-inflammatory effects of thymoquinone(TQ)in in-vivo and in-vitro models of fungal keratitis and the associated mechanisms.Methods The safe anti-inflammatory concentration of TQ was determined using Cell Counting Kit-8 and the in-vivo Draize test with mice.A mouse model of fungal keratitis was established.A subconjunctival in-jection of either 3.75 mg/L TQ or 0.001 dimethyl sulfoxide(DMSO)was given into the mouse cornea.The mouse cornea was exa-mined forevaluating the clinical inflammation score under a microscope.The effect of TQ on neutrophil recruitment in the mouse cornea was examined with hematoxylin-eosin staining and the myeloperoxidase test.The mRNA and protein expression levels of cy-clooxygenase-2(COX-2),interleukin(IL)-1β,and IL-6 in human corneal endothelial cells(HCECs)were measured by quantitative real-time polymerase chain reaction(qRT-PCR)and enzyme-linked immunosorbent assay(ELISA).Results When the concen-tration of TQ was below 3.75 mg/L,the survival rate of HCECs was ≥95％,and sodium fluorescein staining was negative in the cornea of healthy mice,indicating that it was not toxic to HCECs and the mouse cornea.Compared with the DMSO group,the mice in the TQ group showed significantly reduced clinical inflammation scores(F3 d=2.01,P＜0.01;F5 d=3.00,P＜0.01)and signifi-cantly reduced recruitment and activity of neutrophils(t=6.55,P＜0.001).The qRT-PCR and ELISA results showed that com-pared with the DMSO group,HCECs in the TQ group had significantly lower mRNA and protein expression levels of COX-2,IL-1β,and IL-6(t=6.12-15.06,P＜0.001).Conclusion TQ has anti-inflammatory effects against fungal keratitis by reducing neutrophil aggregation and decreasing the expression of inflammatory factors.关键词
百里醌/角膜炎/炎症/烟曲霉菌Key words
thymoquinone/keratitis/inflammation/Aspergillus fumigatus分类
医药卫生引用本文复制引用
姚华,胡丽婷,徐强,刘雪晴,郑雯丹,赵桂秋..百里醌对烟曲霉菌角膜炎的抗炎作用及其机制[J].青岛大学学报（医学版）,2024,60(2):169-173,5.基金项目
国家自然科学基金青年科学基金项目(81900824) （81900824）
