青岛大学学报（医学版）2024，Vol.60Issue(2)：174-179,6.DOI:10.11712/jms.2096-5532.2024.60.065
舌鳞癌组织中性粒细胞对血管生成的作用及其意义
Role of neutrophils in angiogenesis in tongue squamous cell carcinoma tissues
摘要
Abstract
Objective To explore the correlation between microvascular density(MVD)and neutrophil infiltration in oral squamous cell carcinoma(OSCC)of the tongue,and explore the association of MVD with clinicopathological parameters and post-operative survival.Methods MVD and neutrophil infiltration in OSCC tissues were examined by immunohistochemistry with double staining.The relationship between MVD and the clinicopathological features of patients was analyzed.The effect of MVD levels in OSCC tissues on the survival of patients was determined using a Kaplan-Meier survival curve.A Cox regression model was used to analyze independent risk factors affecting the survival of patients with OSCC.The effect of neutrophils on angiogenesis was observed by tube formation assay.Results A higher MVD level in OSCC tissues was significantly associated with a more advanced TNM stage,lymph node metastasis,a larger tumor size,and tumor recurrence(X2=8.161-26.312,P＜0.001).The survival analysis showed that the overall survival and disease-free survival of the high-MVD group were significantly shorter than those of the low-MVD group(X2=7.860,15.270,P＜0.05).The Cox regression analysis showed that MVD,tumor size,and tumor recurrence were independent prognostic factors for the patients with OSCC(β=-1.426-1.468,P＜0.05).The Spearman correlation analysis showed a positive correlation between MVD and the density of neutrophils in OSCC tissues(r=0.352,P＜0.05).The tube formation assay results showed that co-culture with neutrophils promoted the angiogenesis of endothelial cells(t=2.744-4.372,P＜0.05).Conclusion High MVD indicates a poor prognosis in patients with OSCC,which may be a potential prognostic indicator.MVD is correlated with neutrophil infiltration in OSCC tissues,which may promote tumor angiogenesis.关键词
中性白细胞/微血管密度/新生血管化,病理性/头颈鳞癌/预后Key words
neutrophils/microvascular density/neovascularization,pathologic/squamous cell carcinoma of the head and neck/prognosis分类
医药卫生引用本文复制引用
高菲,冯元勇,张旋,李彤彤,王玥琪,王宁..舌鳞癌组织中性粒细胞对血管生成的作用及其意义[J].青岛大学学报（医学版）,2024,60(2):174-179,6.基金项目
国家自然科学基金青年科学基金项目(81702677) （81702677）
