青岛大学学报（医学版）2024，Vol.60Issue(2)：190-194,5.DOI:10.11712/jms.2096-5532.2024.60.066
AA147对Aβ诱导的阿尔茨海默病细胞模型的作用
Effect of AA147 on a cell model of Alzheimer's disease induced by β-amyloid
摘要
Abstract
Objective To investigate whether AA147 can exert a neuroprotective effect on a cell model of Alzheimer's di-sease(AD)by activating the endoplasmic reticulum stress-related protein activating transcription factor 6(ATF6).Methods Cortical neuronsfrom embryonic day 17-19 Sprague-Dawley rat embryos were cultured and induced with β-amyloid(Aβ)1-42 to established a cell model of AD.The cells were divided into control group(neurons pretreated with 10 μmol/L DMSO for 12 h),Aβ1-42 treatment group(neurons induced by 2 μmol/L Aβ1-42 for 24 h),Aβ1-42+DMSO group(neurons pretreated with 10 μmol/L DMSO for 12 h and then induced by 2 μmol/L Aβ1-42 for 24 h),and Aβ1-42+AA147 group(neurons pretreated with 10 μmol/L AA147 for 12 h and then induced by 2 μmol/L Aβ1-42 for 24 h).CCK-8 assay and immunofluorescent staining were used to measure the viabilityof neurons in each group,and Western blot was used to measure the expression of ATF-6 and p-Akt in neurons.Results CCK8 assay showed that there was a significant difference in the viability rate of neurons between groups(F=47.42,P＜0.01);compared with the control group,the Aβ1-42 group had a significant reduction in the viability of neurons(t=9.42,P＜0.05),and compared with the Aβ1-42 group,the Aβ1-42+AA147 group had a significant increase in the viability of neurons(t=5.73,P＜0.05).Immunofluorescent staining showed that compared with the control group,the Aβ1-42 group had a significant reduction in the number of survived neurons,and compared with the Aβ1-42 group,the Aβ1-42+AA147 group had a significant increase in the number of survived neurons,while there was no significant difference between the Aβ1-42+DMSO group and the Aβ1-42 group.Western blot showed that there were significant differences between groups in the protein expression levels of ATF-6 and p-Akt(F=11.77,26.10;P＜0.05);compared with the control group,the Aβ1-42 group had significantly lower pro-tein expression levels of ATF-6 and p-Akt(P＜0.05),and the Aβ1-42+AA147 group had significantly higher expression levels than the Aβ1-42 group(P＜0.05),while there were no significant differences between the Aβ1-42+DMSO group and the Aβ1-42 group(P＞0.05).Conclusion AA147 can exert a neuroprotective effect on the cell model of AD by activating the expression of the ATF-6 and p-Akt signaling pathway.关键词
阿尔茨海默病/内质网应激/激活转录因子6/神经保护Key words
Alzheimer disease/endoplasmic reticulum stress/activating transcription factor 6/neuroprotection分类
医药卫生引用本文复制引用
王慧,侯庆明..AA147对Aβ诱导的阿尔茨海默病细胞模型的作用[J].青岛大学学报（医学版）,2024,60(2):190-194,5.基金项目
国家自然科学基金资助项目(8217051911) （8217051911）
