青岛大学学报（医学版）2024，Vol.60Issue(2)：199-204,6.DOI:10.11712/jms.2096-5532.2024.60.071
载ADSCs的GelMA水凝胶对糖尿病大鼠伤口愈合的作用
Effect of ADSCs-loaded GelMA hydrogel on wound healing in diabetic rats
摘要
Abstract
Objective To investigate the effect of adipose-derived stem cells(ADSCs)-loaded Gelatin Methacrylate(GelMA)hydrogel scaffolds on skin wound healing in diabetic rats.Methods ADSCs were seeded on the surface of GelMA scaf-folds.Streptozocin was used to establish a rat model of diabetes,and a round full-thickness skin defect with a diameter of 2 cm was created on the back of the rats.The rats were divided into control group(without any treatment),GelMA group(treated with scaf-folds alone),and ADSCs-loaded GelMA group(treated with ADSCs-loaded scaffolds).Wound healing was observed for each group;HE staining was used to observe the histology of skin tissue;immunohistochemical staining was used to measure the ex-pression of transforming growth factor-beta 1(TGF-β1).Results Compared with the control group and the GelMA group,the ADSCs-loaded GelMA group had a higher wound healing rate at 14 and 21 days after surgery,a stronger expression of TGF-β1 at 14 days after surgery,and a reduction in capillary bleeding at 21 days after surgery.Conclusion ADSCs-loaded GelMA hydrogel scaffolds can promote wound healing in diabetic rats,and TGF-β1 may play an important role in this process.关键词
伤口愈合/糖尿病并发症/间质干细胞/水凝胶类/组织支架/病理学/大鼠,Sprague-DawleyKey words
wound healing/diabetes complications/mesenchymal stem cells/hydrogels/tissue scaffolds/pathology/rats,Sprague-Dawley分类
医药卫生引用本文复制引用
王玥琪,赵志远,高菲,项锋钢,迟菁华..载ADSCs的GelMA水凝胶对糖尿病大鼠伤口愈合的作用[J].青岛大学学报（医学版）,2024,60(2):199-204,6.基金项目
教育部留学回国人员科研启动基金(80114020109) （80114020109）
