青岛大学学报（医学版）2024，Vol.60Issue(2)：252-256,5.DOI:10.11712/jms.2096-5532.2024.60.075
miR-103a-3p对慢性粒细胞白血病细胞增殖和凋亡影响
Effect of miR-103a-3p on the proliferation and apoptosis of chronic myeloid leukemia cells
摘要
Abstract
Objective To investigate the expression of miR-103a-3p in bone marrow tissue of patients with chronic my-eloidleukemia(CML)and the effect of miR-103a-3p on the proliferation and apoptosis of CML cells.Methods Bone marrow specimens were collected from 32 patients with CML and 20 healthy donors,and quantitative real-time PCR was used to measure the expression of miR-103a-3p.CCK-8 assay and flow cytometry were used to measure the changes in the proliferation and apopto-sis of CML K562 cells,and Western blot was used to measure the expression levels of the apoptosis-related proteins Bcl2-associated X protein(Bax)and B-cell lymphoma-2(Bcl2)in K562 cells.Mice were subcutaneously injected with K562 cells,and hematoxylin-eosin staining was used to observe histopathological changes.Results Compared with the healthy donors,the patients with CML had a significant reduction in the expression level of miR-103a-3p in bone marrow mononuclear cells(t=3.317,P＜0.01),and the expression level of miR-103a-3p in K562 cells was significantly lower than that in normal human bone marrow stroma cells(t=21.430,P＜0.001).In vitro experiments showed that after transfection with agomiR-103a-3p,K562 cells showed significant inhibi-tion of proliferation(t=4.949-12.170,P＜0.01)and a significant increase in apoptosis(t=3.181,P＜0.05).Tumor formation experiments showed that compared with the Lv-miR-NC group,the Lv-miR-up group had a significantly lower tumor mass(t=4.518,P＜0.01)and a significantly smaller tumor volume(t=15.670-36.290,P＜0.001).Conclusion This study shows that miR-103a-3p can inhibit the proliferative capacity of CML K562 cells and promote their apoptosis.关键词
微RNAs/白血病,髓系,慢性,BCR-ABL阳性/K562细胞/细胞增殖/细胞凋亡Key words
microRNAs/leukemia,myelogenous,chronic,BCR-ABL positive/K562 cells/cell proliferation/apoptosis分类
医药卫生引用本文复制引用
王智超,谢文杰,管洪在..miR-103a-3p对慢性粒细胞白血病细胞增殖和凋亡影响[J].青岛大学学报（医学版）,2024,60(2):252-256,5.基金项目
山东省自然科学基金面上项目(ZR2020MH311) （ZR2020MH311）
