青岛大学学报（医学版）2024，Vol.60Issue(2)：261-266,6.DOI:10.11712/jms.2096-5532.2024.60.072
FOXM1靶向HMMR对骨肉瘤细胞凋亡和自噬影响
FOXM1 participates in apoptosis and autophagy of osteosarcoma cells by targeting HMMR
摘要
Abstract
Objective To investigate the effects of forkhead box M1(FOXM1)transcription factor on apoptosis and autopha-gy of osteosarcoma(OS)cells by targeting hyaluronic acid-mediated motor receptor(HMMR).Methods The OS cell lines with FOXM1 knockdown and HMMR overexpression were constructed using the lentiviral vector.The cells transfected with the empty lentivirus were used as the control group,the cells transfected with the sh-FOXM1 lentivirus were used as the FOXM1 knockdown group,and the cells transfected with the sh-FOXM1 lentivirus and OE-HMMR lentivirus were used as the HMMR overexpression group.Western blot was used to detect the expression of FOXM1 in normal osteoblasts(hFOB1.19)and OS cells(U-2OS).The apoptosis rate of U-2OS cells in the control group,FOXM1 knockdown group,and HMMR overexpression group was determined by flow cytometry.Western blot was used to measure the expression of HMMR protein,autophagy protein(LC3 Ⅰ/Ⅱ),and apoptosis-related proteins(Cleaved-Caspase3 and Bax)in the control group and FOXM1 knockdown group,as well as the expres-sion of autophagy protein(LC3 Ⅰ/Ⅱ)in the HMMR overexpression group.Results The expression of FOXM1 in U-2OS cells was significantly higher than that in the hFOB1.19 cells(t=38.780,P＜0.005).Compared with the control group,the FOXM1 knockdown group showed significantly increased apoptosis rate of U-2OS cells(t=3.517,P＜0.05),as well as significantly reduced expression of Cleaved-Caspase3,Bax,LC3 Ⅰ/Ⅱ,and HMMR(t=3.155-6.334,P＜0.05).Overexpression of HMMR restored the increase in apoptosis rate and inhibition of autophagy caused by FOXM1 knockdown(F=31.00,160.20;P＜0.05).Conclu-sion FOXM1 knockdown promotes apoptosis and inhibits autophagy in OS cells by reducing HMMR protein expression.关键词
叉头框蛋白M1/骨肉瘤/透明质酸受体/细胞凋亡/自噬Key words
forkhead box protein M1/osteosarcoma/hyaluronan receptors/apoptosis/autophagy分类
医药卫生引用本文复制引用
翟东昌,唐磊,杨子杰,程明国,张蓓,沈若武..FOXM1靶向HMMR对骨肉瘤细胞凋亡和自噬影响[J].青岛大学学报（医学版）,2024,60(2):261-266,6.基金项目
青岛市自然科学基金项目(22-3-7-smjk-7-nsh) （22-3-7-smjk-7-nsh）
