热带亚热带植物学报2024，Vol.32Issue(3)：417-426,10.DOI:10.11926/jtsb.4814
珍珠芦荟失绿突变体'MT-1'的农艺性状、解剖结构、光合特性和代谢组特征分析
Analysis of Agronomic Traits,Anatomical Structure,Photosynthetic Characters and Metabolomic Characteristics of Leaf Chlorosis Mutant'MT-1'in Aristaloe aristata
摘要
Abstract
In order to elucidate the mutation mechanism of leaf color mutation of Aristaloe aristata,the phenotypic characteristics,leaf ultrastructure,photosynthetic pigment content,chlorophyll fluorescence parameters and metabolomics between leaf color mutant'MT-1'and normal leaf color germplasm'WT-18'were compared.The results showed that compared with'WT-18',the mutant'MT-1'exhibited yellow strips from young leaves,and the plant type became smaller and the growth was slower.The number of stomata became less and small,and the shape changed from rectangle to square.The number of chloroplasts and internal lamella decreased,and the volume decreased obviously.The contents of photosynthetic pigments,including chlorophyll a,chlorophyll b and carotenoids,and chlorophyll fluorescence dynamic parameters(Fo,Fm,Fv/Fm,ФPSⅡ,qP,qN and ETR)decreased significantly.The metabolites of'MT-1'and'WT-18'were significantly different.The KEGG metabolic pathway analysis showed that most of the different metabolites were enriched in amino acid metabolism,glucose metabolism,lipid metabolism,nucleic acid metabolism,secondary metabolite biosynthesis,and other pathways.Therefore,the changes in chloroplast related genes might be an important cause of leaf chlorosis mutation.关键词
珍珠芦荟/突变体/光合色素/叶绿素荧光参数/超微结构/代谢组Key words
Aristaloe aristata/Mutant/Photosynthetic pigment/Chlorophyll fluorescence parameter/Ultra microstructure/Metabolomics引用本文复制引用
陈汉鑫,林艺辉,马馨怡,林秀芳..珍珠芦荟失绿突变体'MT-1'的农艺性状、解剖结构、光合特性和代谢组特征分析[J].热带亚热带植物学报,2024,32(3):417-426,10.基金项目
福建省科技计划项目(农业引导性重点项目)(2020N0060) （农业引导性重点项目）
福建省科技特派员后补助项目(2022S21010074)资助 This work was supported by the Project for Science and Technology Plan in Fujian(Key Project of Agricultural Guiding)(Grant No.2020N0060),and the Project for Science and Technology Special Commissioner Post-Subsidy in Fujian(Grant No.2022S21010074). （2022S21010074）
