四川大学学报（自然科学版）2024，Vol.61Issue(3)：308-316,9.DOI:10.19907/j.0490-6756.2024.036002
响应面优化超声波微波协同提取猕猴桃茎黄酮工艺及抗氧化活性分析
Response surface optimization of ultrasonic-microwave synergistic extraction of flavonoids from Actinidia arguta stems and analysis of antioxidant activity
摘要
Abstract
In order to enhance the utilization efficiency of Actinidia arguta tree resources,the stems of wild Actinidia arguta were used as experimental raw materials for the extraction of total flavonoids using ultrasonic-microwave synergistic extraction.The Plackett-Burman experimental design was employed to evaluate the ultrasonic power and ultrasonic time affecting the extraction efficiency of flavonoids from stems.Seven factors,including ultrasonic power and ultrasonic time,were evaluated,and three of them were se-lected as significant influencing factors:solid-liquid ratio,ultrasonic temperature,and microwave time.On this basis,the Box-Benhnken steepest ascent path method and response surface design were further used to optimize the optimal parameter levels of the three significant influencing factors.The results showed that the extraction rate of flavonoids could reach 9.25%under the conditions of a solid-liquid ratio of 45 mL/g,ultra-sonic temperature of 55 °C and microwave time of 5.5 min.In the measured mass concentration,the scaveng-ing ability of soft jujube kiwi stem flavonoids on DPPH and OH was better than that of VC,while the scav-enging ability of O2-was lower than that of VC,but it had certain scavenging ability for three free radicals.The results showed that the flavonoid extract of Actinidia arguta stem had good antioxidant activity.关键词
软枣猕猴桃茎/超声波微波技术/黄酮/Plackett-Burman试验设计/响应面法/抗氧化Key words
Actinidia arguta stem/Ultrasonic microwave technology/Flavone/Plackett-Burman design/Response surface method/Antioxidation分类
农业科技引用本文复制引用
姜立春,张雨洁,蒋道玉,付丹阳,赵欣悦..响应面优化超声波微波协同提取猕猴桃茎黄酮工艺及抗氧化活性分析[J].四川大学学报（自然科学版）,2024,61(3):308-316,9.基金项目
四川省科技厅自然科学基金项目(2023NSFSC0206) （2023NSFSC0206）
阿坝州科知局应用技术研究与开发资金项目(19YYJSYJ0031) （19YYJSYJ0031）
