类风湿关节炎患者血清lncRNA GATA6-AS1 和GATA6 mRNA水平与疾病活动度指标、免疫功能及疾病严重程度的关系OACSTPCD

Objective To analyze the relationship between long non-coding RNA GATA binding protein 6 antisense RNA1(lncRNA GATA6-AS1)and GATA binding protein 6(GATA6)mRNA levels in serum of rheumatoid arthritis patients and disease ac-tivity indicators,immune function and disease severity.Methods A total of 74 patients in remission stage of rheumatoid arthritis in our hospital from April 2021 to March 2023 were selected as the remission stage group,81 patients in active stage of rheumatoid ar-thritis were selected as active stage group,and another 80 healthy subjects in the same period were selected as control group.General data and disease activity indicators[rheumatoid factor(RF),cell sedimentation rate(ESR),C-reactive protein(CRP),anti-cyclic citrullinated peptide(CCP)antibody,tumor necrosis factor(TNF)-α,28 joint disease activity(DAS28)score]were collected.The ln-cRNA GATA6-AS1 and GATA6 mRNA in serum were detected by fluorescence quantitative PCR.Immune function indexes of the subjects[immunoglobulin(Ig)A,IgM,IgG,CD3+T,CD4+T,CD8+T cells,CD4+/CD8+ratio]were detected.The correlation of serum lncRNA GATA6-AS1,GATA6 mRNA and disease activity indicators,immune function indicators in patients with rheumatoid arthritis,the risk factors affecting the occurrence of patients with rheumatoid arthritis in the active stage,predictive value of serum ln-cRNA GATA6-AS1 and GATA6 mRNA in patients with rheumatoid arthritis during active phase were all analyzed.Results RF,ESR,CRP,anti-CCP antibody,TNF-α,DAS28 score,serum lncRNA GATA6-AS1,GATA6 mRNA,IgA,IgM,IgG,the percent-age of CD3+T,CD4+T,CD8+T cells in peripheral blood and the ratio of CD4+/CD8+were increased in control group,remission stage group and active stage group(P<0.05);lncRNA GATA6-AS1 was positively correlated with GATA6 mRNA in patients with rheumatoid arthritis(P<0.05);serum lncRNA GATA6-AS1 and GATA6 mRNA levels were positively correlated with RF,ESR,CRP,anti-CCP antibody,TNF-α,DAS28 score,IgA,IgM,IgG,CD3+T cells,CD4+T cells,CD8+T cells and CD4+/CD8+ra-tio(P<0.05);RF,ESR,CRP,anti-CCP antibody,TNF-α,DAS28 score,lncRNA GATA6-AS1 and GATA6 mRNA were inde-pendent risk factors affecting the occurrence of patients with rheumatoid arthritis in active stage(P<0.05);Area under curve(AUC)of serum lncRNA GATA6-AS1,GATA6 mRNA and their combination predicted the occurrence of active phase in patients with rheu-matoid arthritis were 0.843,0.861 and 0.928,respectively;the AUC predicted by the combination was greater than that predicted by serum lncRNA GATA6-AS1 and GATA6 mRNA levels separately(P<0.05).Conclusion The serum levels of lncRNA GA-TA6-AS1 and GATA6 mRNA in patients with rheumatoid arthritis are high,and the levels of both are higher in patients with active stage,which are closely related to disease activity indicators,immune function and disease severity,and can well predict the occur-rence of patients with rheumatoid arthritis in active stage.