药学研究2024，Vol.43Issue(5)：417-421,5.DOI:10.13506/j.cnki.jpr.2024.05.001
荔枝核总黄酮通过抑制TLR4-TAK1抗大鼠肝纤维化的机制分析
Mechanism of total flavones from litchi seed against liver fibrosis in rats by inhibiting TLR4-TAK1
摘要
Abstract
Objective To explore the mechanism of anti-hepatic fibrosis effect of total flavones from litchi seed on carbon tetrachloride-induced hepatic fibrosis model.Methods Rat model of hepatic fibrosis was constructed by subcutaneous dorsal injection of 40%carbon tetrachloride-peanut oil solution,and the rats successfully modeled were divided into model group,Fuzheng Huayu group,and total flavones from litchi seed low-,medium-,and high-dose groups,and normal group was also set up.After 8 weeks of continuous gavage administration,the left lobe of the liver was taken for HE staining to observe the histopathological changes in the liver;The expression levels of Col Ⅰ,Col Ⅲ,and α-SMA mRNA in the liver of the rats in each group were detected by RT-qPCR;And the expression of TLR4,TAK1,P-TAK1,JNK,and P-JNK in the liver of the rats was detected by Western blotting.Results HE staining of liver tissues showed that all doses of total flavones from litchi seed improved the inflammatory infiltration of rat liver to different degrees when compared with that of rats with liver fibrosis model.The detection of liver fibrosis-related factors showed that the expression of Col Ⅰ,Col Ⅲ and α-SMA mRNA in the livers of rats at all doses of total flavones from litchi seed was significantly decreased(P<0.05 or P<0.01).The results of Western blotting showed that the expression of liver TLR4 and P-TAK1 proteins in the low and medium dose groups of total flavones from litchi seed was significantly decreased(P<0.05)compared with that in the model group,and the hepatic P-JNK protein in the low dose group was also significantly decreased(P<0.05).Conclusion Total flavones from litchi seed attenuated carbon tetrachloride-induced hepatic fibrosis in rats,and this effect may be related to the inhibition of hepatic TLR4-TAK1 pathway.关键词
荔枝核总黄酮/肝纤维化/Toll样受体-4/转化生长因子β激活激酶1Key words
Total flavones from litchi seed/Liver fibrosis/TLR4/TAK1分类
中医学引用本文复制引用
覃田田,黄盼玲,胡兴兰,罗伟生,李宇清..荔枝核总黄酮通过抑制TLR4-TAK1抗大鼠肝纤维化的机制分析[J].药学研究,2024,43(5):417-421,5.基金项目
国家自然科学基金项目(No.82160834) （No.82160834）
广西壮瑶药重点实验室开放课题(No.GXZYZZ2022-15) （No.GXZYZZ2022-15）
广西中医药大学桂派杏林拔尖人才项目(No.2022C007) （No.2022C007）
广西中医药大学岐黄工程高层次人才团队培育项目(No.202405):中药学广西一流学科(No.桂教科研[2022]1 号) （No.202405）
