胆汁制钩吻总碱抑制结肠癌细胞增殖的作用研究OACSTPCD

Objective To investigate the in vitro anti-tumor effect of the alkaloids of bile-processed Gelsemium elegans(ABPG)and provide scientific basis for the study of the post-detoxification efficacy of Gelsemium elegans(GE).Methods The proliferation effects of ABPG on human colon cancer HCT-116 cells,human glioblastoma U87 cells,human hepatocellular carcinoma HepG2 cells,and human lung adenocarcinoma A549 cells were assessed using CCK-8 assay.Subsequently,focusing on colon cancer HCT-116 cells,different concentrations of ABPG(50,100,200 μg·mL-1)were used to intervene with the cells.The influence on cell cycle arrest was determined by flow cytometry.Cell apoptosis was detected using Annexin V FITC/PI flow cytometry assay.Furthermore,investigate apoptosis-related protein expression from a protein level.Results At the IC50 concentration of GE,the proliferation inhibitory rate of ABPG on U87,A549,HepG2,and HCT-116 tumor cells was higher than that of the GE group,and the differences between the groups were statistically significant(P<0.01).Compared with the control group,treatment with different concentrations of ABPG(50,100,200 μg·mL-1)effectively reduced colony formation of HCT-116 cells and arrested the cell cycle in the G2/M phase.ABPG also induced late-stage apoptosis in colon cancer HCT-116 cells and regulated apoptosis-related proteins Bax,Bcl-2,and caspase-3.Conclusion ABPG can suppress the proliferation of colon cancer HCT-116 cells by regulating the expression of cell cycle and apoptosis-related proteins.