山东农业科学2024，Vol.56Issue(5)：36-41,6.DOI:10.14083/j.issn.1001-4942.2024.05.005
小麦四个抗白粉病基因的KASP标记检测
KASP Marker Detection of Four Powdery Mildew Resistant Genes in Wheat
摘要
Abstract
In order to improve detection efficiency of Pm13,and to know the utilization status of four mildew resistant genes as Pm21,PmV,Pm12 and Pm13 in wheat breeding programs of Shandong Province,KASP marker of Pm13 was successfully transformed by PCR sequencing and primer designing.The KASP markers of the four genes were employed to detect 140 lines and 2 control cultivars of wheat in the 2022-2023 regional trails of Shandong,belonging to high yield group and high quality group.The result showed that none of the four genes were detected in any of the 140 materials,which indicated that these genes were rarely used in wheat breeding of Shandong Province.This study could provide references for KASP marker-assisted selec-tion of wheat powdery mildew resistant genes and promote their utilization in wheat breeding.关键词
小麦/抗白粉病基因/Pm13/KASP标记/分子标记辅助选择Key words
Wheat/Powdery mildew resistant gene/Pm13/KASP marker/Marker-assisted selection分类
农业科技引用本文复制引用
李玮,宋国琦,张迎迎,李玉莲,张淑娟,张荣志,李吉虎,高洁,李根英..小麦四个抗白粉病基因的KASP标记检测[J].山东农业科学,2024,56(5):36-41,6.基金项目
山东省农业科学院农业科技创新工程项目(CXGC2023A01) （CXGC2023A01）
山东省重点研发计划项目(2019LZGC009,2022LZGCQY002) （2019LZGC009,2022LZGCQY002）
