山东农业科学2024，Vol.56Issue(5)：132-137,6.DOI:10.14083/j.issn.1001-4942.2024.05.017
小菜蛾幼虫生防菌株毒力测定及产毒条件优化
Virulence Determination and Optimization of Toxin Production Conditions of Biocontrol Strains of Plutella xylostella Larvae
摘要
Abstract
To enrich microbial resources for biological control of Plutella xylostella,this study was con-ducted using three fungi strains as Clonostachys rosea,Purpureocillium lilacinum and Bionectria sp.02 with good biocontrol effects.Their virulence to the larvae of P.xylostella was tested,and the strain with high viru-lence was selected,then its virulence was determined and the toxin production conditions were optimized.The results showed that the three strains were all effective in controlling the larvae of P.xylostella,and the most ef-fective one was C.srosea.The virulence test showed that the half lethal time(LT50)and half lethal concentra-tion(LC50)of C.rosea were 30.88 h and 4.365×104 spores per milliliter.According to the optimization results of toxin production conditions of C.rosea,the optimal medium was SDAY,the optimal pH value was 6,and the optimal culture time was 7 days.In summary,C.rosea had the characteristics of high virulence and rapid pathogenicity to the larvae of P.xylostella,so it had great biocontrol potential and could be used as an excel-lent biocontrol fungus against P.xylostella.关键词
小菜蛾幼虫/生防菌株筛选/毒力测定/粉红粘帚霉/产毒条件优化Key words
Plutella xylostella L./Screening of biocontrol strains/Virulence determination/Clonostachys rosea/Optimization of toxin production conditions分类
农业科技引用本文复制引用
张梓月,芦俊佳,杨明国,马云强,田芸菁,勾朝金,刘勉..小菜蛾幼虫生防菌株毒力测定及产毒条件优化[J].山东农业科学,2024,56(5):132-137,6.基金项目
云南省科研基金项目"十字花科蔬菜害虫小菜蛾生防菌株筛选及毒力测定"(2023Y0751) （2023Y0751）
云南省农业联合专项"薇甘菊入侵监测数据融合方法与高精度预警模型研究"(202101BD070001-128) （202101BD070001-128）
云南省"兴滇英才支持计划"青年人才项目(990123069) （990123069）
云南农业联合专项"粉红粘帚霉对楚雄腮扁叶蜂致病机理研究"(202301BD070001-091) （202301BD070001-091）
