饲料研究2024，Vol.47Issue(9)：106-112,7.DOI:10.13557/j.cnki.issn1002-2813.2024.09.019
响应面法优化提取壮药三七姜总酚酸及其镇痛抗炎作用机制研究
Optimization of response surface method for the extraction of Zhuang medicine Stahlianthus involucratus total phenolic acids and its analgesic and anti-inflammatory mechanism
摘要
Abstract
Response surface method(RSM)was used to optimize the extraction process of Stahlianthus involucratus total phenolic acids and explore its analgesic and anti-inflammatory mechanism.Total phenolic acids were extracted from Stahlianthus involucratus by reflux extraction.The extraction process was optimized by single factor test and Box-Behnken test,and the effects of ethanol concentration,liquid-to-material ratio,and extraction time on the yield of total phenolic acids were explored.Writhing model and cotton ball granuloma model of mice induced by acetic acid were established to study the analgesic and anti-inflammatory mechanism of total phenolic acids in Stahlianthus involucratus.The results showed that the optimum extraction process of total phenolic acids from Stahlianthus involucratus were an ethanol concentration of 42％,liquid-material ratio of 60 mL/g,and reflux time of 60 min.Under these conditions,the yield of total phenolic acids of Stahlianthus involucratus can reach 6.09 mg/g.Compared with the model group,each dose of ethanol extract of Stahlianthus involucratus had an inhibitory effect on granuloma inflammation,reduced the number of writhing,decreased the contents of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),prostaglandin E2(PGE2),interleukin-1β(IL-1β),5-hydroxytryptamine(5-HT)and increased the content of cyclic adenosine monophosphate(cAMP).The study indicates that the optimized extraction process of Stahlianthus involucratus by response surface methodology is stable and reliable,and can be used for the extraction of total phenolic acids from Stahlianthus involucratus.Stahlianthus involucratus has certain analgesic and anti-inflammatory activities,and its mechanism may be related to reducing the secrete of pro-inflammatory cytokines such as IL-6,TNF-α,IL-1β,and PGE2 in vivo.关键词
三七姜/响应面法/总酚酸/镇痛/抗炎Key words
Stahlianthus involucratus/response surface method/total phenolic acids/analgesic/anti-inflammation分类
农业科技引用本文复制引用
刘星晨,梁洁,马丽娟,曹玉嫔,陈嘉怡,陈晓思,魏江存,赵立春..响应面法优化提取壮药三七姜总酚酸及其镇痛抗炎作用机制研究[J].饲料研究,2024,47(9):106-112,7.基金项目
广西自然科学基金项目(项目编号:2020GXNSFAA238026) （项目编号:2020GXNSFAA238026）
国家中医药管理局高水平中医药重点学科建设项目-少数民族药学(壮药学)(项目编号:zyyzdxk-2023165) （壮药学）
广西中医药重点学科壮药学(项目编号:GZXK-Z-20-64) （项目编号:GZXK-Z-20-64）
广西一流学科中药学(民族药学)(项目编号:桂教科研[2018]12号) （民族药学）
广西高等学校千名中青年骨干教师培育计划(项目编号:桂教人[2019]5号) （项目编号:桂教人[2019]5号）
广西中医药大学大学生创新创业训练计划项目(项目编号:202310600156) （项目编号:202310600156）
广西中医药大学第三批"岐黄工程"高层次人才团队培育项目(项目编号:202406) （项目编号:202406）
国家青年岐黄学者项目(项目编号:2020-7) （项目编号:2020-7）
