CRISPR-Cas9基因编辑技术及其在家禽中的研究进展OA北大核心CSTPCD

The CRISPR-Cas system,found in bacteria or archaea,serves as an adaptive immune system to counteract viral re-invasion by effectively cleaving nucleic acids.By leveraging the characteristics of Cas9 protein and its variants,researchers have devised a range of gene editing techniques that enable gene operations like gene knock-out or knock-in within cells,thereby facilitating genetic diversity in organisms.Additionally,these tools allow the manipulation of DNA or RNA through epigenetic modification to regulate gene expression,rendering them extensively employed in life science research.Poultry,as a significant agricultural species,plays a crucial role in supplying individuals with protein of superior quality.The utilization of CRISPR-Cas9 technology for gene editing and modification in avian genome is presently confined to the research phase,with remarkable advancements observed solely in chicken and quail.This paper presents an overview of CRISPR-Cas9 gene editing technology,encompassing its components,delivery methods,and optimization strategies,while also exploring its potential applications in poultry production and prevention and control of diseases.These insights lay the groundwork for the future development of CRISPR-Cas9 technology in the context of poultry system.