生物技术通报2024，Vol.40Issue(5)：225-236,12.DOI:10.13560/j.cnki.biotech.bull.1985.2023-1088
不同多年生稻品种内生细菌群落多样性比较分析
Comparison and Analysis of Endophytic Bacterial Communities in Different Perennial Rice Varieties
摘要
Abstract
[Objective]To explore the structure and potential functions of endophytic bacterial microbiota in perennial rice,as well as the relationship between endophytic bacteria in perennial rice and host agronomic traits.[Method]The diversity,composition and metabolic function of endophytic bacteria in different in perennial rice varieties(PR23,PR25,and PR107)were characterized via Illumina sequencing of the 16S rRNA gene.[Result]These results showed that the beta diversity of endophytic bacterial communities significantly differed among the distinct genotypes of perennial rice.Due to the differentiation of tissue structures,perennial rice varieties shaped distinctive ecological niches for endophytes in different tissue compartments.Proteobacteria,Firmicutes and Bacteroidetes were the dominant endophytic bacterial phyla in the underground tissues of perennial rice,while Proteobacteria,Actinobacteria and Firmicutes were the dominant phyla in the aboveground tissues.These perennial rice varieties had different endophytic bacterial taxa with specific amplicon sequence variants(ASVs),dominant genera and specific biomarkers.The endophytic bacterial communities of perennial rice were reassembled for various metabolic functions,including biosynthesis,degradation/utilization/assimilation,detoxification,generation of precursor metabolite and energy,glycan pathways,macromolecule modification and metabolic clusters.Significantly different MetaCyc pathways of endophytic bacteria in perennial rice varieties with distinct genotypes were involved in the biosynthesis and degradation of esters,alcohols and polysaccharides.[Conclusion]These results indicated host genotypes and tissues differentiation affect the community structure,species composition and metabolic function of endophytic bacteria in perennial rice.关键词
多年生稻/内生细菌/群落结构/16S rRNA测序Key words
perennial rice/endophytic bacteria/community structure/16S rRNA sequencing引用本文复制引用
孔德婷,齐笑含,刘兴蕾,李丽萍,胡凤益,黄立钰,秦世雯..不同多年生稻品种内生细菌群落多样性比较分析[J].生物技术通报,2024,40(5):225-236,12.基金项目
云南省基础研究重点项目(202101AS070001),云南大学研究生科研创新项目(YAS202309260077-1,ZC-22222674,ZC-23234490) （202101AS070001）
