长链非编码RNA CYP1B1-AS1对急性髓系白血病细胞增殖和迁移的影响及机制实验研究OACSTPCD

Objective:To explore the effect and mechanism of long non-coding RNA(lncRNA)CYP1B1-AS1 on proliferation and migration of acute myeloid leukemia cells.Methods:RT-qPCR was used to detect the expression of CYP1B1-AS1 in acute myeloid leukemia cell lines THP-1,NB4,HL-60,KG-1,SKM-1 and bone marrow stromal cells HS-5.Acute myeloid leukemia cell lines were treated with 5-Aza-CdR,and the effect of 5-Aza-CdR on CYP1B1-AS1 was detected by RT-qPCR.HL-60 cells were divided into NC group and CYP1B1-AS1 group according to different transfectants.Clone formation experiment and scratch experiment were used to detect the proliferation and migration ability of HL-60 cells.Dual-luciferase experiment was used to verify the targeting relationship between CYP1B1-AS1 and miR-1306-5p.RT-qPCR was used to detect the effect of CYP1B1-AS1 on the expression of miR-1306-5p in HL-60 cells.Western blot was performed to detect the effect of CYP1B1-AS1 on the expressions of CDK2,Cyclin E,Twist and Fibronectin in HL-60 cells.Results:Compared with HS-5 cells,the expression of CYP1B1-AS1 was de-creased in acute myeloid leukemia cell lines,and the expression of CYP1B1-AS1 was the lowest in HL-60 cell line(all P＜0.05).5-Aza-CdR could significantly promote the expression of CYP1B1-AS1 in acute myeloid leukemia cell lines(all P＜0.05).Compared with the NC group,the proliferation ability and migration rate of HL-60 cells in the CYP1B1-AS1 group were reduced(all P＜0.05).Overexpression of miR-1306-5p could inhibit the luciferase activity of wild-type CYP1B1-AS1(P＜0.05).Compared with the NC group,the expression of miR-1306-5p in HL-60 cells in the CYP1B1-AS1 group was decreased,and the expression of cell proliferation and migration related proteins CDK2,Cyclin E,Twist and Fibronectin were decreased(all P＜0.05).Conclusion:Overexpression of CYP1B1-AS1 may inhibit the proliferation and migration of acute myeloid leukemia cells by targeted down-regulation of miR-1306-5p expression.