长链非编码RNA RP11-497E19.1对胃癌细胞增殖和侵袭的影响及机制实验研究OACSTPCD

Objective:To investigate the effect of lncRNA RP11-497E19.1 on the proliferation and invasion of gastric cancer cells and its mechanism.Methods:RT-qPCR was used to detect the expression of RP11-497E19.1 in gastric cancer cell lines HS-746T,BGC823,NCI-N87,SGC7901,AGS and immortalized gastric epithelial cell line GES-1,and the cells with the highest expression were selected for subsequent experiments.HS-746T cells were divid-ed into si-NC group and si-RP11-497E19.1 group,which were transfected with negative control oligonucleotide or RP11-497E19.1 small interfering RNA,respectively.The proliferation and invasion ability were analyzed by colony formation assay and Transwell assay.The targeting relationship between RP11-497E19.1 and miR-545-5p was veri-fied by dual luciferase reporter gene assay.The expression of miR-545-5p was detected by RT-qPCR.The expression of c-Met/BVR/ATF-2 pathway related proteins were detected by Western blot.Results:Compared with GES-1 cells,the expression levels of RP11-497E19.1 in HS-746T,BGC823,NCI-N87,SGC7901 and AGS cells were increased,and the expression level of RP11-497E19.1 in HS-746T cells was the highest(all P＜0.05).Compared with the si-NC group,the proliferation and invasive number of HS-746T cells in the si-RP11-497E19.1 group were decreased(all P＜0.05).Dual luciferase reporter gene assay confirmed that RP11-497E19.1 targeted miR-545-5p and negatively regulated the expression of miR-545-5p(P＜0.05).Compared with the si-NC group,the expression of c-Met,BVR,p-ATF-2,Snail1 and Snail2 proteins in the c-Met/BVR/ATF-2 molecular pathway in HS-746T cells in the si-RP11-497E19.1 group decreased(all P＜0.05).Conclusion:RP11-497E19.1 is highly expressed in gastric cancer cells.Silencing RP11-497E19.1 inhibits the proliferation and invasion of gastric cancer HS-746T cells by targeting the miR-545-5p/c-Met/BVR/ATF-2 molecular pathway.