口服银耳多糖对自然衰老小鼠皮肤的改善作用及机制实验研究OACSTPCD

Objective:To investigate the effect of tremella fuciformis polysaccharide(TFPS)on the skin of natu-rally aging mice and its possible mechanism.Methods:Seventy-five female KM mice aged 12 months were randomly divided into aging model group(group M),TP-100 group[100 mg/(kg·d)],TP-200 group[200 mg/(kg·d)]and TP-400 group[400 mg/(kg·d)]and hyaluronic acid(HA)group[200 mg/(kg·d)],15 rats in each group.Different doses of TFPS and HA were added to the drinking water of the experimental group every week for 12 weeks.Another 15 female KM mice aged 2 months were used as young control group(group Y).At the end of the intervention,the skin of the back of the mice was taken,and the skin physical and chemical properties,oxidative stress indexes(CAT,T-AOC,GSH-Px),endogenous components(HA,Col Ⅰ,Col Ⅲ)and collagen degradation in-dexes(MMP-1,MMP-3,MMP-9)were detected.Results:Compared with group Y,the skin moisture content was significantly decreased in M group(P＜0.05).Compared with group M,the skin moisture content was increased in HA group and each dose of TFPS group(all P＜0.05).Compared with group Y,the skin weight was significantly decreased in group M(P＜0.05).Compared with the M group,the skin weight was increased in the HA and TP-400 groups(both P＜0.05).Compared with group Y,the levels of GSH-Px,CAT and T-AOC were significantly de-creased in group M(all P＜0.05).Compared with group M,GSH-Px was increased in HA,TP-100 and TP-200 groups,CAT was increased in TP-400 group,and T-AOC was increased in HA group and each dose of TFPS group(all P＜0.05).The contents of Col Ⅰ,Col Ⅲ and HA were significantly lower in group M than those in group Y(all P＜0.05).Compared with group M,the content of Col Ⅰ in skin was increased in TP-100 and TP-400 groups,the content of Col Ⅲ in skin was increased in HA group and each dose of TFPS group,and the content of HA in skin was increased in TP-400 group(all P＜0.05).Compared with group Y,the contents of MMP-3 and MMP-9 were signifi-cantly increased in group M(all P＜0.05).The contents of MMP-3 and MMP-9 in skin of each dose of TFPS group and HA group were significantly different from those in group M(all P＜0.05).Conclusion:Oral administration of TFPS can delay natural skin aging in mice possibly by enhancing the activity of antioxidant enzymes and reducing the production of oxygen free radicals to inhibit the activity of MMP in the skin,thereby reducing the loss of skin collagen.