陕西医学杂志2024，Vol.53Issue(6)：739-743,5.DOI:10.3969/j.issn.1000-7377.2024.06.004
基质金属蛋白酶12在胃癌组织、细胞中的表达及对裸鼠移植瘤生长的影响实验研究
Expression of matrix metalloproteinase-12 in gastric cancer tissues and cells and its effect on growth of transplanted tumor in nude mice
摘要
Abstract
Objective:To investigate the expression of matrix metalloproteinase-12(MMP-12)in gastric cancer tissues and gastric cancer cell line SGC-7901 and its effect on the growth of transplanted tumor in nude mice.Meth-ods:Forty-two cases of patients with gastric cancer were collected,and the tumor tissues and normal gastric mucosa tissues(＞3 cm from the edge of the tumor)were collected after surgery,and immunohistochemical EnVision meth-od was used to detect the expression of MMP-12.The sh-MMP12 plasmid was synthesized and transfected into SGC-7901 by lentiviral vector,which was set as sh-MMP12 group,and the cells without any intervention were set as blank control group(NC group),and the expressions of MMP-12 and PCNA protein were detected by Western blot,and the expressions of MMP-12 and PCNA mRNA were detected by RT-qPCR.The cell suspension of sh-MMP12 group and NC group were injected into the left and right armpits of 5 BALB/C female nude mice(the left was sh-MMP12 group and the right was NC group),respectively,and the tumor volume was measured and recorded at an interval of 7 days,and the mice were sacrificed after 28 days to weigh the tumor.Results:The results of immunohistochemistry showed that the positive rate of MMP-12 in gastric cancer tissues was higher than that in normal gastric mucosa tis-sues,and the expression of MMP-12 was positively correlated with PCNA in gastric cancer tissues(all P＜0.05).Western blot results showed that the expressions of MMP-12 and PCNA protein in sh-MMP12 group were signifi-cantly lower than those in NC group(all P＜0.05).RT-qPCR results showed that the expressions of MMP-12 and PCNA mRNA in the sh-MMP12 group were lower than those in the NC group(all P＜0.05).The tumor volume of sh-MMP12 group was not significantly different from that of NC group at 7 and 14 days(all P＞0.05),but was sig-nificantly smaller than that of NC group at 21 and 28 days(all P＜0.05);after 28 days,the weight of axillary tumor in sh-MMP12 group was lower than that in NC group(P＜0.05).Conclusion:The expression of MMP-12 is in-creased in gastric cancer tissues and SGC-7901 cell line,and inhibition of MMP-12 can slow down the growth of transplanted tumor in nude mice.关键词
胃癌/基质金属蛋白酶12/增殖细胞核抗原/裸鼠/免疫组化/移植瘤Key words
Gastric cancer/Matrix metalloproteinase-12/Proliferating cell nuclear antigen/Nude mice/Immu-nohistochemistry/Transplanted tumor分类
医药卫生引用本文复制引用
冀增秋,顾洪柱,李佳琳,王莹,张子玉..基质金属蛋白酶12在胃癌组织、细胞中的表达及对裸鼠移植瘤生长的影响实验研究[J].陕西医学杂志,2024,53(6):739-743,5.基金项目
辽宁省科学技术计划项目(2021-BS-268) （2021-BS-268）
