陕西医学杂志2024，Vol.53Issue(6)：744-747,753,5.DOI:10.3969/j.issn.1000-7377.2024.06.005
基于Wnt/β-连环蛋白通路探究金天格对肿瘤坏死因子-α诱导的小鼠MC3T3E1细胞生物学功能的影响实验研究
Effect of Jintiange on biological function of mouse MC3T3E1 cells induced by tumor necrosis factor-α based on Wnt/β-catenin pathway
摘要
Abstract
Objective:To investigate the effec of Jintiange on the biological function of mouse osteoblasts(MC3T3E1)induced by tumor necrosis factor-α(TNF-α)by regulating Wnt/β-catenin pathway.Methods:MC3T3E1 cells were cultured in vitro and divided into control group,TNF-α group(50 ng/ml TNF-α),L-Jintiange group(50 ng/ml TNF-α+10-6 g/L Jintiange),M-Jintiange group(50 ng/ml TNF-α+10-5 g/L Jintiange),H-Jintiange group(50 ng/ml TNF-α+10-4 g/L Jintiange),DKK-1 group(50 ng/ml TNF-α+10 ng/ml Wnt/β-catenin pathway inhibitor DKK-1),H-Jintiange+LiCl group(50 ng/ml TNF-α+10-4 g/L Jintiange+20 μmol/L Wnt/β-catenin pathway activator LiCl).CCK-8 assay kit was applied to detect cell activity.Flow cytometry was applied to detect cell apoptosis.Cellular IL-1β and IL-6 levels were measured by ELISA.Western blot was applied to detect the expression of apoptosis related proteins and Wnt/β-catenin signaling pathway proteins.Results:Compared with the control group,the cell viability,the protein expression of Bcl-2,PD-L1 in TNF-α group were obviously reduced,and apoptosis rate,the levels of IL-1β and IL-6,the expression of Bax,β-catenin,TCF7L2 and Cyclin D1 proteins were significantly elevated(all P＜0.05).Compared with the TNF-α group,the cell viability,the protein expression of Bcl-2 and PD-L1 in the L-Jintiange group,M-Jintiange group,H-Jintiange group,and DKK-1 group were obviously increased,and the apoptosis rate,the levels of IL-1β andL-6,the expression of Bax,β-catenin,TCF7L2,and Cyclin D1 proteins were ob-viously reduced(all P＜0.05).Compared with the H-Jintiange group,the cell viability,the expression of Bcl-2,and PD-L1 proteins in the H-Jintiange+LiCl group were obviously reduced,and the apoptosis rate,the levels of IL-1β andIL-6,the expression of Bax,β-catenin,TCF7L2 and Cyclin D1 proteins were obviously increased(all P＜0.05).Con-clusion:Jintiange may alleviate TNF-α-induced damage to MC3T3E1 cells by inhibiting the Wnt/β-catenin pathway.关键词
金天格/肿瘤坏死因子-α/MC3T3E1细胞/Wnt/β-catenin通路/细胞增殖/细胞凋亡/小鼠Key words
Jintiange/Tumor necrosis factor-α/MC3T3E1 cells/Wnt/β-catenin pathway/Cell proliferation/Cell apoptosis/Mouse分类
医药卫生引用本文复制引用
张婷,刘丹,贠丹丹,耿男..基于Wnt/β-连环蛋白通路探究金天格对肿瘤坏死因子-α诱导的小鼠MC3T3E1细胞生物学功能的影响实验研究[J].陕西医学杂志,2024,53(6):744-747,753,5.基金项目
陕西省自然科学基础研究计划项目(2021JZ-59) （2021JZ-59）
西安医学院第一附属医院横向基金资助项目(HX-2022-01) （HX-2022-01）
