沈阳医学院学报2024，Vol.26Issue(3)：231-236,6.DOI:10.16753/j.cnki.1008-2344.2024.03.002
粪菌移植对单侧输尿管梗阻小鼠肾脏的保护作用
Protective effect of fecal microbiota transplantation on kidney of mice with unilateral ureteral obstruction
摘要
Abstract
Objective:To investigate the effect of fecal microbiota transplantation(FMT)on unilateral ureteral obstruction(UUO)-induced renal fibrosis in mice.Methods:Male C57BL/6J mice were randomly divided into the control group,UUO group,UUO+FMT group and myeloid-derived suppressor cells(MDSCs)transfer group.The latter 3 groups were constructed renal fibrosis models by UUO,the UUO+FMT group received FMT from control mice after UUO surgery,and the MDSCs transfer group underwent splenectomy and intravenous injection of MDSCs from the peripheral blood of the UUO+FMT group.Renal tissue specimens were collected at 14 d after UUO for HE and Masson staining.TNF-α and IL-10 expression levels were detected by real-time fluorescence quantitative PCR(qRT-PCR),Western blot and immunofluorescence staining.Results:HE staining showed that the renal tissue structure of the control group was normal.The UUO group showed significant dilatation of renal tubules accompanied by obvious inflammatory cell infiltration.However,the renal lesions in the UUO+FMT group and MDSCs transfer group were significantly improved compared with those in the UUO group.Masson staining showed that the positive area of collagen deposition significantly increased in the UUO group compared with the control group,while the positive area of collagen deposition decreased in the UUO+FMT group and MDSCs transfer group compared with the UUO group.The results of qRT-PCR,Western blot and immunofluorescence showed that the expression levels of TNF-α and IL-10 were elevated in renal tissues of the UUO group compared with the control group.Compared with the UUO group,the expression level of TNF-α decreased while IL-10 increased in the UUO+FMT group and MDSCs transfer group.Conclusions:FMT reduces the expression of TNF-α and increase the expression of IL-10 in the kidney of UUO mice,and alleviates renal fibrosis.FMT may exert a nephroprotective effect by regulating MDSCs.关键词
粪菌移植/肾纤维化/输尿管梗阻/肠道菌群Key words
fecal microbiota transplantation/renal fibrosis/ureteral obstruction/intestinal flora分类
医药卫生引用本文复制引用
王雅洁,肖梓豪,曹玉涵..粪菌移植对单侧输尿管梗阻小鼠肾脏的保护作用[J].沈阳医学院学报,2024,26(3):231-236,6.基金项目
国家自然科学基金青年基金项目(No.81702092) （No.81702092）
安徽省高校优秀青年科研项目(No.2023AH030107) （No.2023AH030107）
