特产研究2024，Vol.46Issue(3)：56-59,4.DOI:10.16720/j.cnki.tcyj.2023.021
龙岩山麻鸭胚胎成纤维细胞的培养和鉴定
Culture and Identification of Embryo Fibroblasts from the Longyan Shan-Ma Duck
摘要
Abstract
To cultivate the duck embryo fibroblasts from Longyan Shan-Ma duck.The 10-day-old duck embryo tissues of Longyan Shan-Ma duck were used for the fibroblast culture by the tissue block method and enzyme digestion method,and the cultured fibroblasts were identified by the immunofluorescence method.The results indicated that the cultured duck embryo fibroblasts showed a typical long spindle shape after 12 h,and the outline of the cells were well-defined and the nucleoli were distinct after 48 h.The 3rd generation fibroblasts were all positive after DAPI and green fluorescence staining with the characteristic of fibroblasts.Fibroblasts were successfully isolated and cul-tured from the embryo tissue of the Longyan Shan-Ma duck,which provides good materials for the late research of molecular biology and cell biology of the Longyan Shan-Ma duck.关键词
龙岩山麻鸭/鸭胚成纤维细胞/细胞培养/免疫荧光Key words
Longyan Shan-Ma duck/duck embryo fibroblast/cell culture/immunofluorescence分类
农业科技引用本文复制引用
吴恺琪,吴琼,王蕾,向仕玺,林明昕,吴忠亮..龙岩山麻鸭胚胎成纤维细胞的培养和鉴定[J].特产研究,2024,46(3):56-59,4.基金项目
福建省科技厅引导性项目(2020N0034) （2020N0034）
