特产研究2024，Vol.46Issue(3)：137-143,7.DOI:10.16720/j.cnki.tcyj.2023.068
固相萃取结合UPLC-MS/MS法测定陈皮中6种黄酮类成分
Extraction Phase Ultra Performance Liquid Chromatography-tandem Mass Spectrometry
摘要
Abstract
An analytical method was developed for the determination of six flavonoids in Citri Reticulatae Pericarpium based on the solid phase extraction by UPLC-MS/MS.The samples were purified by HLB-D101 dual cartridge solid phase extraction.Chromatographic col-umn-Hypersil Gold C18 column(100 mm × 2.1 mm,1.9 μm),was used with 0.1％formic acid-acetonitrile as the mobile phase.Six flavo-noids were detected by electrospray ionization mass spectrometry in positive and negative switching with MRM mode.The results showed that the selected dual solid phase extraction packing could effectively separate and purify 6 flavonoids from CRP.By optimizing the chro-matographic and mass spectrometry parameters,the four flavanones of naringin,narirutin,hesperidin and neohesperidin showed good line-arity in the concentration range of 0.05～10 μg/mL in negative ion mode,and the detection limit was 5 mg/kg.In the positive ion mode,the linear ranges of citrine and citrine were 0.005～1.0 μg/mL,and the detection limit was 0.lmg/kg.The average recoveries were 92.5％～107.5％with relative standard deviations(RSD)of 1.7％～6.4％.The established method is rapid and efficient,with a high sensitivity,good recovery and reproducibility in this study.The results showed that the content of flavonoids in CRP of the same variety was relatively stable,and there were obvious differences in some flavonoids among different varieties.关键词
黄酮类/超高效液相色谱/质谱/固相萃取/陈皮Key words
flavonoid/ultra performance liquid chromatography/mass spectrometry/solid phase extraction/Citri Reticulatae Pericarpium分类
医药卫生引用本文复制引用
陈树东,梁土金,冯霁,胡文军,鲁澄宇..固相萃取结合UPLC-MS/MS法测定陈皮中6种黄酮类成分[J].特产研究,2024,46(3):137-143,7.基金项目
广东省药品监督管理局科技创新项目(2021YDZ08) （2021YDZ08）
广东省药品监督管理局重点实验室项目(2022ZDB13) （2022ZDB13）
广东省级农村科技特派员重点派驻任务项目(KTP20200127) （KTP20200127）
