天津中医药大学学报2024，Vol.43Issue(5)：424-433,10.DOI:10.11656/j.issn.1673-9043.2024.05.07
乌梅木瓜汤对菌群失调小鼠肠道菌群及消化酶活性的影响
Effects of Wumei Papaya Decoction on the gutmicrobiota and enzyme activity in mice with dysbiosis
摘要
Abstract
[Objective]To study the effects of Wumei Papaya Decoction on gut microbiota and enzyme activity of antibiotic-induced dysbiosis mice.[Methods]The gut dysbiosis mice model was induced by gavage of mixed antibiotic solution.The changes of gut microbiota of mice induced by Wumei Papaya Decoction were detected by 16S rRNA sequencing technology,and the enzyme activity in feces was measured by Folin and DNS method.[Results]Compared with the normal group,the gutmicrobiota diversity of the model group was significantly reduced,Proteobacteria,Verrucomicrobiota,Klebsiella,Escherichia-Shigella were significantly increased,Firmicutes,Desulfobacterota,norank_f_Muribaculaceae,Lactobacillus was significantly reduced,and the activity of protease,amylase and cellulase were significantly increased,and the activities of protease,amylase and cellulase were significantly increased.Compared with the model group,the intervention of Wumei Papaya Decoction significantly improved the alpha diversity and beta diversity of mice with dysbiosis,and significantly reduced the enzyme activity.The Wumei Papaya Decoction can significantly increase the abundance of beneficial bacteria such as norank_f_Muribaculaceae and Lactobacillus,and reduce the community abundance of conditional pathogenic bacteria such as Proteobacteria,Klebsiella,Escherichia-Shigella.[Conclusion]Wumei Papaya Decoction can regulate gut microbial diversity and enzyme activity.It is speculated that gut microbiota and digestive enzyme activities may be the potential targets of Wumei Papaya Decoction in improving gastrointestinal function.关键词
乌梅木瓜汤/肠道菌群/高通量测序/消化酶Key words
Wumei Papaya Decoction/gut microbiota/high-throughput sequencing/digestive enzyme分类
医药卫生引用本文复制引用
张紫千,席素曼,苗小雨,赵森,王亚静,赵鑫..乌梅木瓜汤对菌群失调小鼠肠道菌群及消化酶活性的影响[J].天津中医药大学学报,2024,43(5):424-433,10.基金项目
国家自然科学基金资助项目(81803959) （81803959）
国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTD-C-202009) （ZYYCXTD-C-202009）
天津市科技计划项目(19ZYPTJC00060,18ZXXYSY00130). （19ZYPTJC00060,18ZXXYSY00130）
