无机材料学报2024，Vol.39Issue(5)：517-524,8.DOI:10.15541/jim20230464
小粒径Fe3O4-DMSA-PEI磁性纳米颗粒的制备及其基因负载能力研究
Fe3O4-DMSA-PEI Magnetic Nanoparticles with Small Particle Size:Preparation and Gene Loading
摘要
Abstract
Ferroferric oxide(Fe3O4)magnetic nanoparticles are widely used as passive targeting carriers in gene therapy,due to their simple preparation,targeting under external magnetic field and easy surface grafting.This study synthesized oil phase Fe3O4 nanoparticles with controllable particle sizes in the range from 4 to 9 nm by regulating the accumulation growth time in the solvothermal method.Then,meso-2,3-dimercaptosuccinic(DMSA)was employed to double exchange oleic acid molecules on its surface to provide good water dispersibility.Finally,Fe3O4-DMSA-PEI magnetic nanoparticles were obtained by grafting branched polyethylenimine(PEI)onto Fe3O4-DMSA surface through amidization reaction.The results demonstrate that the Fe3O4-DMSA-PEI magnetic nanoparticles have a surface Zeta potential of(52.50±1.94)mV,remaining a certain degree of superparamagnetism(14.48 emu/g,1 emu/g=1 A·m2/kg).When the mass ratio of Fe3O4-DMSA-PEI magnetic nanoparticles to plasmid DNA is 15∶1,it can completely block DNA and its loading capacity is as high as 6.67％.The Fe3O4-DMSA-PEI magnetic nanoparticles prepared in this study have a certain gene delivery ability and are expected to be used as gene carriers in the field of gene transfection.关键词
四氧化三铁/磁性纳米颗粒/基因载体/表面改性Key words
ferroferric oxide/magnetic nanoparticle/gene carrier/surface modification分类
通用工业技术引用本文复制引用
蔡和庆,韩璐,杨松松,薛新玉,张扣,孙志成,刘儒平,胡堃,危岩..小粒径Fe3O4-DMSA-PEI磁性纳米颗粒的制备及其基因负载能力研究[J].无机材料学报,2024,39(5):517-524,8.基金项目
国家自然科学基金(61971049,62211530446,22278037) （61971049,62211530446,22278037）
北京印刷学院科研创新团队项目(Ea202205) （Ea202205）
北京市高等教育学会2023年立项重点课题(ZD202312)National Natural Science Foundation of China(61971049,62211530446,22278037) （ZD202312）
Scientific Research Innovation Project from Beijing Institute of Graphic Communication(Ea202205) （Ea202205）
The 2023 Key Project from Beijing Association of Higher Education(ZD202312) （ZD202312）
