武警医学2024，Vol.35Issue(5)：395-398,4.
爱帕琳肽13对GK大鼠BMSCs骨向分化及线粒体呼吸功能的影响
Effect of Apelin-13 on osteoblast differentiation and mitochondrial respiration function of BMSCs derived from GK diabetic rats
摘要
Abstract
Objective To investigate the effect of Apelin-13 on osteoblast differentiation and mitochondrial respiration function of BMSCs derived from GK diabetic rats.Methods The BMSCs of GK diabetic rats were extracted.After cultured in osteogenic differentiation medium,the activity of alkaline phosphatase(ALP)was detected.Von Kossa staining was used to evaluate calcium nodules formation,and the mitochondrial respiratory function under different states was determined by O2K mitochondrial respiration apparatus.Results Apelin-13 enhanced the ALP activity of BMSCs in GK diabetic rats,and promoted calcium nodules mineralization.Compared with the GK group,Ape-lin-13 effectively improved mitochondrial respiratory function in two states:complex I state 3 respiration(16.1±1.8)vs.(5.6±0.9)]pmol/(s×ml)and complex Ⅰ+complex Ⅱ state 3 respiration[(71.1±8.6)vs.(49.0±4.8)]pmol/(s×ml).Conclusions Apelin-13 can promote osteoblast differentiation and enhance mitochondrial respiration function of BMSCs derived from GK diabetic rats.关键词
爱帕琳肽13/骨髓间充质干细胞/线粒体呼吸Key words
Apelin-13/BMSCs/mitochondrial respiration分类
医药卫生引用本文复制引用
李梦,李爽,张欣然,朱彪,郑雪,王坚..爱帕琳肽13对GK大鼠BMSCs骨向分化及线粒体呼吸功能的影响[J].武警医学,2024,35(5):395-398,4.基金项目
北京市医管局青年项目(QML20230809) （QML20230809）
