武警医学2024，Vol.35Issue(5)：421-424,4.
髓磷脂蛋白零样蛋白1对膀胱癌细胞增殖、迁移和侵袭的影响
Effects of MPZL1 on proliferation,migration and invasion of bladder cancer cells
夏连杰 1付文强 1刘逸飞 1张晓蕾 1王保存 1李玉柱2
作者信息
- 1. 063008,唐山中心医院泌尿外科
- 2. 100039 北京,解放军总医院第三医学中心泌尿外科
- 折叠
摘要
Abstract
Objective To investigate the effects of myelin protein zero like 1(MPZL1)on proliferation,migration and inva-sion of bladder cancer T24 cells.Methods The expression of MPZL1 in bladder cancer T24 cells was knocked down by siRNA trans-fection to form an experimental model of bladder cancer cells that inhibited the expression of MPZL1.The cells were divided into si-NC group and si-MPZL1 group.The expression of MPZL1 gene in bladder cancer patients in TCGA database was analyzed online through ualcan database.The changes of cell proliferation were detected by CCK-8 assay and plate cloning assay,and the changes of cell mi-gration and invasion were detected by scratch assay and Transwell assay.Results The MPZL1 gene was highly expressed in patients with bladder cancer and the expression level showed an increasing trend with the progress of clinical stage.The high expression of MP-ZL1 gene was significantly correlated with the shortened survival time of bladder cancer.Inhibition of MPZL1 expression could effec-tively inhibit the proliferation,migration and invasion of bladder cancer T24 cells.Conclusions Knocking down the expression of MP-ZL1 in bladder cancer T24 cells can inhibit the proliferation,migration and invasion of bladder cancer cells.关键词
膀胱癌/髓磷脂蛋白零样蛋白1/增殖/迁移/侵袭Key words
bladder cancer/myelin protein zero like 1/proliferation/migration/invasion分类
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夏连杰,付文强,刘逸飞,张晓蕾,王保存,李玉柱..髓磷脂蛋白零样蛋白1对膀胱癌细胞增殖、迁移和侵袭的影响[J].武警医学,2024,35(5):421-424,4.
