西北林学院学报2024，Vol.39Issue(3)：10-17,8.DOI:10.3969/j.issn.1001-7461.2024.03.02
松材线虫泛素结合酶基因家族生物信息学与表达分析
Identification and Bioinformatic Analysis of the Ubiquitin-Conjugating Enzyme in Bursaphelenchus xylophilus
摘要
Abstract
Pine wood disease caused by Bursaphelenchus xylophilus poses a serious threat to pine species in several countries and regions such as East Asia and Europe.Ubiquitin conjugating enzymes(UBCs)play an important role in various aspects of organism growth,development,and response to stress by mediating the proteasomal degradation of target proteins.In this study,we analyzed the genome of the pine wood nema-tode to identify 20 members of the UBC family.Subsequently,we conducted bioinformatics analysis to ex-plore the physicochemical properties,protein structures,and expression levels of the identified UBC genes during the development of the pine wood nematode.Chromosomal mapping results revealed that the UBC genes are evenly distributed on all chromosomes except for chromosome 5.Topological analysis indicated that most of these genes are located intracellularly.Gene structure analysis showed that UBC genes mainly consist of 2 to 8 exons.The exon-intron structure is highly conserved among species and correlates with the amino acid sequence polymorphism,also exhibiting codon bias on exons.Gene expression data demonstra-ted differential expression of members within the UBC family during different growth and developmental stages.By employing bioinformatics and gene expression analysis methods,this study provides preliminary insights into the role of UBCs in the growth and development process of the pine wood nematode.These findings are of significant guidance and theoretical value for understanding the developmental mechanism of the pine wood nematode and constructing molecular targets for its control and prevention.关键词
松材线虫/泛素结合酶家族/基因鉴定/基因表达分析Key words
Bursaphelenchus xylophilus/ubiquitin conjugating enzyme(UBC)/gene identification/gene expression analysis分类
农业科技引用本文复制引用
赫传杰,戴文昊,潘佳亮..松材线虫泛素结合酶基因家族生物信息学与表达分析[J].西北林学院学报,2024,39(3):10-17,8.基金项目
国家重点研发计划(2021YFD1400900). （2021YFD1400900）
