现代妇产科进展2024，Vol.33Issue(6)：451-455,5.DOI:10.13283/j.cnki.xdfckjz.2024.06.005
抗磷脂抗体损害子宫蜕膜血管内皮细胞血管形成的机制研究
Study on mechanism of antiphospholipid antibody damage to the angiogenesis capacity of decidual vascular endothelial cells
摘要
Abstract
Objective:To study the effect of antiphospholipid antibody(aPL)on the proliferation,migration and angiogenesis of decidual vascular endothelial cells(DVECs).Methods:DVECs were identified by immunofluorescence assay.CCK-8 assay was used to detect the effect of aPL on DVECs proliferation.The effect of aPL on DVECs migration and angiogene-sis was observed by cell scratch assay and angiogenesis assay.Real-time PCR(RT-PCR)was used to detect the effect of aPL on MMP-2 and VEGF mRNA expression in DVECs.Western blot was used to detect the effect of aPL on ERK phosphorylation,VEGF and MMP-2 protein ex-pression in DVECs.Results:aPL inhibited the proliferation,migration and angiogenesis of DVECs.aPL can inhibit ERK phosphorylation,VEGF and MMP-2 expression in DVECs.The expression levels of p-ERK,VEGF and MMP-2 in DVEC cells were significantly increased after the addition of ERK agonists.Conclusion:aPL can reduce the expressions of VEGF and MMP-2 in DVECs by regulating ERK pathway,and then inhibit the proliferation,migration and angio-genesis of DVECs.关键词
抗磷脂抗体/蜕膜组织血管内皮细胞/ERK通路/血管形成Key words
Antiphospholipid antibody/Decidual vascular endothelial cells/ERK path-way/Angiogenesis分类
医药卫生引用本文复制引用
张琳琳,郑雪梅,王谢桐,董艳..抗磷脂抗体损害子宫蜕膜血管内皮细胞血管形成的机制研究[J].现代妇产科进展,2024,33(6):451-455,5.基金项目
国家自然科学基金面上项目(No:81873842) （No:81873842）
临沂市重点研发计划(医学类)(No:2022YX0092) （医学类）
