肾组织石蜡切片Ⅳ型胶原α链免疫组织化学染色在诊断Alport综合征中的应用OA

Objective Immunohistochemical detection of type Ⅳ collagen alpha chain in paraffin sec-tions of renal puncture tissues from patients with Alport syndrome(AS)was performed to explore its clinical application.Methods Kidney tissues from 10 AS patients(AS case group),10 cardiac death organ donor(DCD)donor kidneys and 10 patients diagnosed with immunoglobulin A nephropathy(control group)diag-nosed in Kidney Disease Center,the First Affiliated Hospital of Zhejiang University School of Medicine from November 2018 to July 2022 were selected for fully automated immunohistochemical staining of type Ⅳ colla-gen α1,α3 and α5 chains in paraffin sections,which were compared with the results of immunofluorescence staining of frozen sections.The paraffin sections of renal tissues from the control group were subjected to anti-genic repair by various methods respectively,and the repair effects were compared.Results In the control group,the two staining methods of renal tissue,type Ⅳ collagen α1,α3 and α5 chains staining,showed continu-ous linear positive expression in the glomerular basement membrane(GBM).The two staining methods of re-nal tissue of the AS case group.Type Ⅳ collagen α1 chain staining,showed continuous linear positive expres-sion in the GBM,and α3,α5 chains staining showed negative or weakly positive expression in the GBM.The staining results of the two staining methods were in high consistency(Kappa=0.615,P=0.035).Conclusion Fully automated immunohistochemical detection of type Ⅳ collagen α1,α3 and α5 in paraffin sections can be used for diagnosis of AS better,which provides a reliable technique for the diagnosis and study of AS.