畜牧兽医学报2024，Vol.55Issue(5)：2022-2031,10.DOI:10.11843/j.issn.0366-6964.2024.05.020
雄激素受体抑制剂恩杂鲁胺对山羊卵泡颗粒细胞增殖凋亡的影响
The Effect of the Androgen Receptor Inhibitor Enzalutamide on Proliferation and Apoptosis of Goat Ovarian Granulosa Cells
摘要
Abstract
The aim of this study was to investigate the effects of androgen receptor(AR)on goat granulosa cell proliferation and apoptosis using enzalutamide.Firstly,granulosa cells from goats were isolated and subjected to in vitro culture.The suitable concentration of enzalutamide for cell culture was determined by microscopic observation and CCK8 assay.The experiment was then divided into 3 groups:blank control group(no treatment),negative control group(treated with DMSO),and experimental group(treated with enzalutamide).Each group was set up with 3 replicates.Cell proliferation was detected using the EDU assay,while cell apoptosis was assessed using Caspase3 activity and apoptosis detection kits.The expression of genes and proteins related to cell proliferation and apoptosis was evaluated using quantitative real-time PCR(qRT-PCR)and Western blot analysis.The results showed that the AR inhibitor,enzalutamide,significantly inhibited cell proliferation and reduced the mRNA levels of CCND1,PCNA,and MKI67 in granulosa cells.It also suppressed the protein expression of CCND1,PCNA,and β-catenin.Additionally,enzalutamide promoted apoptosis in goat granulosa cells,as evidenced by increased mRNA levels of BAX and BCL2,without affecting their protein expression.Furthermore,enzalutamide,while not affecting the mRNA level of CASP3,enhanced Caspase3 activity.These findings suggest that enzalutamide,by inhibiting AR activity,can modulate the expression of β-catenin,PCNA,CCND1,Caspase 3,and other genes involved in the regulation of granulosa cell proliferation and apoptosis.关键词
山羊/颗粒细胞/AR/恩杂鲁胺/增殖凋亡Key words
goat/granulosa cell/androgen receptor/enzalutamide/proliferation apoptosis分类
农业科技引用本文复制引用
董书餐,毛帅翔,伍翠莹,李耀坤,孙宝丽,郭勇庆,邓铭,刘德武,柳广斌..雄激素受体抑制剂恩杂鲁胺对山羊卵泡颗粒细胞增殖凋亡的影响[J].畜牧兽医学报,2024,55(5):2022-2031,10.基金项目
广东省林业科技创新项目(2023KJCX004) （2023KJCX004）
广东省南方现代草牧业(羊)创新团队(2023KJ127) （羊）
广东省农业农村厅项目(2022-XPY-00-009) （2022-XPY-00-009）
韶关市仁化县牛羊产业园项目(GDSCYY2021-018) （GDSCYY2021-018）
