畜牧兽医学报2024，Vol.55Issue(5)：2146-2153,8.DOI:10.11843/j.issn.0366-6964.2024.05.032
竞争ELISA和间接ELISA方法应用于牛布鲁氏菌病净化的研究
Study on Purification of Bovine Brucellosis by Competitive ELISA and Indirect ELISA
摘要
Abstract
The aim of this study was to evaluate the efficacy of the combination of competitive ELISA and indirect ELISA methods in the purification of brucellosis,hoping to provide technical support for the prevention and control of brucellosis.A total of 3 271 bovine serum samples from a cattle farm in the northwest of China were tested using the competitive ELISA(cELISA)antibody detection kit,the indirect ELISA(iELISA)antibody detection kit,and the micro-complement fixation test(mCFT)which developed by the writers team.This study adopted a purification strategy of cELISA for preliminary screening,iELISA for confirm diagnosis.The positive cattle were eliminating,the suspect cattle and negative cattle were respectively quarantined after disinfecting completely.After the previous detection,the group was sampled every month for multiple"Detection-Elimination"cycles.When the individual positive rate was below 2％or all negative,the results were verified using a micro-complement fixation test(mCFT).The test were carried on for 2 months after all the bovine turned negative to ensure the successful of the purification.The results showed that,after the implementation of this purification strategy,the positive rate of the infected group with a positive rate of 35.36％decreased to 25.41％in the first month,7.16％in the second month,and 1.86％in the third month.By the fourth month,the group had achieved a comprehensive negative conversion,and the negative rate by mCFT verification was 100％.The detection continued for 2 months,and the individual positive rate was 0.So far,the brucellosis purification of the infected group was realized,making nearly half of the cattle in the group free from culling,greatly reducing the economic loss.In conclusion,cELISA is suitable for the preliminary screening,while iELISA is suitable for confirmed diagnosis of brucellosis.The combination of two methods,through multiple consecutive tests,can achieve a comprehensive purification of brucellosis infected within a relatively short period of time.关键词
布鲁氏菌病/竞争酶联免疫吸附试验/间接酶联吸附试验/微量补体结合试验/初筛/确诊Key words
brucellosis/competitive ELISA/indirect ELISA/micro-complement fixation test/preliminary screening/confirmed diagnose分类
农业科技引用本文复制引用
赵灿奇,冯宇,吕浪,李彦军,魏玉磊,丁家波,陈祥,蒋卉..竞争ELISA和间接ELISA方法应用于牛布鲁氏菌病净化的研究[J].畜牧兽医学报,2024,55(5):2146-2153,8.基金项目
少数民族专业人才特殊培养计划项目 （）
江苏省人兽共患病学重点实验室资助项目(R2210) （R2210）
