畜牧兽医学报2024，Vol.55Issue(5)：2154-2167,14.DOI:10.11843/j.issn.0366-6964.2024.05.033
西藏牦牛源牛支原体T10株全基因组测序及其序列分析
Whole Genome Sequencing and Sequence Analysis on T10 of Mycoplasma bovis Strain from Yaks in Xizang
摘要
Abstract
This study aims to further improve the complete genome database of Mycoplasma bovis,elucidate its biological characteristics and genetic evolution relationship,the whole genome sequencing and sequence analysis of Mycoplasma bovis T10 strain derived from Xizang yaks were conducted.The entire genome of T10 strain was sequenced using the Nanopore and Illumina PE150 sequencing platforms,and the genomic characteristics were analyzed using bioinformatics.Genetic phylogenetic trees were used to compare genetic evolution relationships with domestic and foreign isolates.The gene sequencing results showed that the total genome size of T10 strain was 987 812 bp,with a GC content of 29.27％.It was predicted that 822 coding genes were present,with a total length of 709 129 bp;According to the annotation results of the functional database,22 genes related to membrane transport proteins,7 genes related to carbohydrate enzymes,4 genes related to glycosyltransferases,43 genes related to secretion proteins,51 genes related to T3SS effector proteins,28 genes related to pathogen host interaction,14 genes related to bacterial virulence,and 10 genes related to resistance were annotated;Through comparative genomic analysis,it was found that T10 and 08M,CQ-W70,Hubei-1,Ningxia-1,and PG45 all exhibit amino acid mutations and gene fragment insertions or deletions,as well as differences in the number of gene families,with a difference in the number of gene families ranging from 8 to 32.The results of genetic evolution analysis show that T10 is in the same branch as HB0801,16M,Hubei-1,CQ-W70,NM2012,Ningxia-1,08M,JF4278,KG4397,and PG45,but in different branches from PG1 and PG2.Among them,T10 has the closest genetic relationship with HB0801 and the farther genetic relationship with PG45.This study not only improved the complete genome database of Mycoplasma bovis and elaborated on the genes related to pathogenicity,but also fully elucidated the genetic evolution relationship between T10 strain and other Mycoplasma bovis strains at home and abroad.It clarified the basic genome information of T10 strain and its genetic relationship with domestic and foreign strains,providing a theoretical basis for future research on pathogenic mechanism vaccines.关键词
牦牛/牛支原体/全基因组测序/基因功能注释/比较基因组Key words
yak/Mycoplasma bovis/whole-genome sequencing/gene function annotation/comparative genomics分类
农业科技引用本文复制引用
罗婷,韩著,徐业芬,蔡林,索朗斯珠,徐晋花,牛家强..西藏牦牛源牛支原体T10株全基因组测序及其序列分析[J].畜牧兽医学报,2024,55(5):2154-2167,14.基金项目
西藏自治区重点研发计划项目(XZ202001ZY0046N) （XZ202001ZY0046N）
财政部和农业农村部"国家现代农业产业技术体系"项目(CARS-37) （CARS-37）
西藏农牧学院研究生教育创新计划项目(YJS2023-20) （YJS2023-20）
