畜牧兽医学报2024，Vol.55Issue(5)：2168-2175,8.DOI:10.11843/j.issn.0366-6964.2024.05.034
荚膜B型多杀性巴氏杆菌外膜囊泡生物学特性分析与免疫效果评价
Biological Characteristics and Immune Effect Evaluation of Outer Membrane Vesicles of Capsular Type B Pasteurella multocida
摘要
Abstract
In order to explore the biological characteristics and immune effect of outer membrane vesicles(OMVs)of capsular type B Pasteurella multocida.Outer membrane vesicles were extracted by ultra-high speed and density gradient centrifuge methods.The shape and size of the outer membrane vesicles were observed by transmission electron microscope(TEM),the proteins of OMVs were analyzed by SDS-PAGE and LC-MS/MS,the protein concentration was determined by the BCA method,and the endotoxin content was determined by limulus lysate test.Antibody titers and immune protection test were proceeded in New Zealand white rabbits.The results showed that OMVs had a regular spherical structure,the average particle size was 108.3 nm.OMVs contained a variety of protein bands,the protein bands were mainly 33,40,53 ku.The endotoxin content was 3.31×106 EU·mL-1.The serum antibody titer of immunized New Zealand white rabbits was 1∶405 600 when the injection dose was 50 μg per rabbit.The transcription levels of IFN-γ and IL-4 in peripheral blood lymphocytes increased.The immune protection efficiency against capsular tybe B Pasteurella maltocida was 75％.In conclusion,the OMVs of capsular type B Pasteurella multocida have good immunoprotective effcet and has the potential to be an candidate vaccine.This study lay the foundation for the research of OMVs vaccines.关键词
多杀性巴氏杆菌/外膜囊泡/生物学特性/疫苗Key words
Pasteurella multocida/outer membrane vesicles/biological characteristics/vaccine分类
农业科技引用本文复制引用
高洁,李晓成,穆杨,张慧,魏荣,李劼..荚膜B型多杀性巴氏杆菌外膜囊泡生物学特性分析与免疫效果评价[J].畜牧兽医学报,2024,55(5):2168-2175,8.基金项目
国家重点研发计划(2021YFD1800300) （2021YFD1800300）
