稀释比例对绵羊精子活率、活力、运动速率、质膜完整率和顶体完整率的影响OA北大核心CSTPCD

This study was to determine the optimal dilution ratio for preserving sheep semen at 15℃and to achieve a better semen preser-vation effect.The semen samples were diluted at ratios of 1∶1,1∶4,1∶9,1∶14,1∶19,and 1∶24,respectively.Then,total sperma-tozoa motility,progressive motility,straight line velocity(VSL),curvilinear velocity(VCL),average path velocity(VAP),amplitude of lateral head displacement(ALH),average motion degree(MAD),plasma membrane integrity,and acrosome integrity of the samples were measured during preservation.The results showed that the total spermatozoa motility in the 1∶9 dilution group was significantly(P＜0.05)higher than that in the other dilution ratio groups,for 3-6 days of preservation.The progressive spermatozoa motility in the 1∶4 and 1∶9 di-lution groups was significantly higher than those in the other dilution ratio groups,for 1-5 days of preservation(P＜0.05).During3-5 days of preservation,the plasma membrane integrity and acrosome integrity were significantly higher in the 1∶4 and 1∶9 dilution groups,com-pared with the other dilution groups(P＜0.05).During 3-6 days of preservation,VCL,VAP,and ALH in the 1∶4 and 1∶9 dilution groups were significantly higher than those in the other dilution ratio groups(P＜0.05).However,the difference between the two groups was not significant(P＞0.05).Therefore,based on the conditions of this experiment,the most appropriate dilution ratio for preserving sheep se-men at 15℃was 1∶9.Both excessively high and low dilution ratios were found to be unfavorable for sheep semen preservation.