畜牧与兽医2024，Vol.56Issue(6)：72-77,6.
犬支气管肺泡灌洗术建立及灌洗液细胞学统计分析
Development of bronchoalveolar lavage and cytological analysis of lavage fluid in dogs
摘要
Abstract
To establish standardized BAL procedures,BALF cytological testing procedures,and a standard database for healthy dogs in or-der to provide reference for the clinical application of bronchoalveolar lavage through fiberoptic bronchoscopy in veterinary medicine,in this study,14 healthy Beagle dogs with no signs of respiratory diseases were selected for multiple bronchoscopic alveolar lavage.BALF was recov-ered and the recovery rate was calculated.The total nucleated cell count(TNCC),cell classification counts and statistical analyses were per-formed.Atlas of endoscopic bronchial anatomy in healthy dogs was performed by BAL manipulation.The results were that the qualified rate of the samples in 98 BAL was 92.1%,and the probability of bleeding was 3.4%.In the 89 qualified samples,the highest BAL recovery rates were found in the right accessory lobe(64.5%),the posterior left cranial lobe(60.4%)and the anterior left cranial lobe(57.7%).The average quantity of the nucleated cells in BALF was 304.48×106/L,and the frequency of the cell types from high to low were that of macro-phages(85.9%),lymphocytes(13.0%),neutrophils,mast cells and plasma cells;while the proportion of epithelial cells was strongly af-fected by human manipulation,and was generally ignored in classification and counting.In this study,the atlas of endoscopic bronchial anat-omy in healthy dogs was drawn,the BAL operation procedure and the reference value of BALF cytology were established,which would be of help for the diagnosis and differentiation of canine lower respiratory diseases in veterinary clinic practice.关键词
支气管镜/支气管肺泡灌洗/细胞学/呼吸系统Key words
bronchoscopy/bronchoalveolar lavage/cytology/respiratory system分类
农业科技引用本文复制引用
陈小竹,盛正尧,沈嘉仪,杨德吉,姚大伟..犬支气管肺泡灌洗术建立及灌洗液细胞学统计分析[J].畜牧与兽医,2024,56(6):72-77,6.基金项目
中央高校基本科研业务费项目(KYYJ202104) （KYYJ202104）
