畜牧与兽医2024，Vol.56Issue(6)：130-138,9.
7种中药提取物体外抑制金黄色葡萄球菌的效果及机制研究
The effect and mechanism of seven traditional Chinese medicine extracts on in vitro inhibition of Staphylococcus aureus
摘要
Abstract
The aim of this study was to investigate the in vitro antibacterial effects of seven Chinese herbal medicines,namely,baicalin,berberine,Rhododendron,Saxifraga stolonifera Meerb,Dracocephalum tanguticum Maxin,Terminalia chebula Retz.,and Hippophae rham-noides on Staphylococcus aureus and to explore their potential antibacterial mechanisms.The minimum inhibitory concentrations of the different herbal medicines against Staphylococcus aureus were determined using the micro-broth dilution method,and the in vitro inhibitory effects of these herbal medicines on Staphylococcus aureus were studied by measuring the growth curves of Staphylococcus aureus.Then,the effect of the herbal medicines on the biofilm production,biofilm permeability and intracellular adenosine triphosphate(ATP)content of Staphylococcus aureus were determined to reveal the potential antibacterial mechanisms.The results showed that all the herbal extracts had in-hibitory effects on Staphylococcus aureus,among which berberine and baicalin presented better inhibitory effects than other herbal extracts.The growth of Staphylococcus aureus in the herbal treatment group was slow,and berberine exhibited the strongest growth inhibition effect.The aqueous extracts of berberine,baicalin,Tangut Cyanine Blue,Azadirachta indica,Sea-buckthorn fruit,and Tiger grass,and the alco-holic extracts of Tangut Cyanine Blue,Azadirachta indica,Chebul,Sea-buckthorn fruit,and Tiger grass,significantly inhibited the formation of Staphylococcus aureus biofilm.After treatment with baicalin and berberine,the total extracellular protein and nucleic acid con-tents of Staphylococcus aureus increased in positive correlation with the concentration of drug extracts.The intracellular ATP content of Staph-ylococcus aureus treated with berberine decreased significantly.Taken together,the seven herbal medicines had in vitro antibacterial effects on Staphylococcus aureus and were able to inhibit bacterial growth by the mechanisms of affecting biofilm permeability and cellular energy metab-olism of Staphylococcus aureus.关键词
中药提取物/抑菌效果/生物膜/细胞膜通透性/ATPKey words
herbal extracts/antibacterial effect/biofilms/cell membrane permeability分类
畜牧业引用本文复制引用
刘俊俊,田佳仪,李坤,毕崇亮,韩照清,秦建华..7种中药提取物体外抑制金黄色葡萄球菌的效果及机制研究[J].畜牧与兽医,2024,56(6):130-138,9.基金项目
河北省研究生创新能力培养资助项目(CXZZBS2023075) （CXZZBS2023075）
