刑事技术2024，Vol.49Issue(3)：229-236,8.DOI:10.16467/j.1008-3650.2024.0012
HPLC-MS/MS法测定尿液中的氟胺酮及其代谢物
Determination of 2-fluoro-deschloroketamine and Its Metabolites in Urine by HPLC-MS/MS
摘要
Abstract
In this paper,the specific structures of two first-phase metabolites of 2-fluoro-deschloroketamine except nor-2-fluoro-deschloroketamine were determined,and a detection method of 2-fluoro-deschloroketamine,nor-2-fluoro-deschloroketamine,cis-Dihydro-2-fluoro-deschloroketamine and trans-Dihydro-2-fluoro-deschloroketamine in urine was estabished using HPLC-QQQ MS/MS.Through HPLC-HRMS screening,two metabolites of 2-fluoro-deschloroketamine,namely cis-Dihydro-2-fluoro-deschloroketamine and trans-Dihydro-2-fluoro-deschloroketamine were determined and then were synthesized specifically.The structures were identified by 1H NMR,13C NMR and 2D NOE NMR.The HPLC-QQQ MS/MS method for the detection of 2-fluoro-deschloroketamine,nor-2-fluoro-deschloroketamine,cis-Dihydro-2-fluoro-deschloroketamine and trans-Dihydro-2-fluoro-deschloroketamine in urine was established,which possesses a good linear relationship for 2-fluoro-deschloroketamine and its three main metabolites at the concentration range of 0.5～200 ng/mL,with the linear correlation coefficients all greater than 0.996 3,and the recoveries ranging from 81.60％to 119.33％.The quantitation of 2-fluoro-deschloroketamine and its three metabolites in actual urine samples was detected by the established method.This study determined the specific structures of two first-phase metabolites of 2-fluoro-deschloroketamine.The developed testing method has low detection limit,high recovery,and good reproducibility,which can be used in the qualitative and quantitative detection for the stable metabolites of 2-fluoro-deschloroketamine.关键词
法医毒物学/氟胺酮/核磁/顺式氟胺醇/反式氟胺醇/高效液相色谱-三重四极杆串联质谱Key words
forensic toxicology/2-fluoro-deschloroketamine/nuclear magnetic resonance(NMR)/cis-Dihydro-2-fluoro-deschloroketamine/trans-Dihydro-2-fluoro-deschloroketamine/high performance liquid chromatography-triple quadrupole mass spectrometry分类
社会科学引用本文复制引用
马俊,李冬梅,夏梦婵,陈军,赵一哲,万耔莘,朱孔文,赵霞,郑珲..HPLC-MS/MS法测定尿液中的氟胺酮及其代谢物[J].刑事技术,2024,49(3):229-236,8.基金项目
国家自然科学基金青年科学基金项目(22104149) （22104149）
