刑事技术2024，Vol.49Issue(3)：318-322,5.DOI:10.16467/j.1008-3650.2023.0068
DNATyper™ 21与Verifiler™ Plus试剂盒对常规案件检材检验的比较
Comparative Evaluation of DNATyper™ 21 and Verifiler™ Plus Kit in Forensic DNA Casework
摘要
Abstract
The DNATyper™ 21 amplification kit is a 5-dye multiplex assay,which is widely used in construction of the Chinese National autosomal DNA database.However,the DNATyper™ 21 kit has not been extensively used in forensic DNA casework.Therefore,this study aimed to elucidate the reliability of the DNATyper™ 21 kit compared with Verifiler™ Plus kit and to assess its applicability on forensic analysis.Standard DNA samples with concentrations of 0.031 25,0.062 5,0.125,0.25,0.5 and 1.0 ng/μL were used for sensitivity testing.DNATyper™ 21 kit and Verifiler™ Plus kit were utilized to detect the DNA extracted from 1056 casework evidence samples and the allele consistency of the same sample was analyzed.The results showed that both kits could successfully detect standard DNA with a template concentration above 0.062 5 ng/μ.L.For casework evidence samples,DNATyper™ 21 kit successfully detected 881 cases out of 1 056,and the detection rate is 83.4％,while Verifiler™ Plus kit successfully detected 892 cases out of 1 056,and the detection rate is 84.5％.The statistical results showed that there was no significant difference in the detection rate between the two kits,and the alleles obtained at the same locus of the same sample were completely consistent.In conclusion,the DNATyper™ 21 is considered to have the capability to be used for STR analysis of casework evidence samples.关键词
法医物证学/DNATyper™ 21试剂盒/Verifiler™Plus试剂盒/比较研究/生物检材Key words
forensic biology/DNATyper™ 21 kit/Verifiler™ Plus kit/comparative evaluation/casework evidence samples分类
社会科学引用本文复制引用
王剑,马云龙,余政梁,龚政,高馨玉,杨亮,张丽娜..DNATyper™ 21与Verifiler™ Plus试剂盒对常规案件检材检验的比较[J].刑事技术,2024,49(3):318-322,5.基金项目
法医遗传学公安部重点实验室开放课题基金资助项目(2020FGKFKT03) （2020FGKFKT03）
