医学信息2024，Vol.37Issue(11)：79-83,5.DOI:10.3969/j.issn.1006-1959.2024.11.014
质谱分析小鼠神经母细胞瘤细胞外泌体的蛋白组成
Mass Spectrometry Analysis of Protein Composition of Exosomes from Mouse Neuroblastoma Cells
摘要
Abstract
Objective To understand the proteins composition of exosomes and the functions of the proteome.Methods The exosomes of mouse neuroblastoma cells were obtained by ultracentrifugation,and the protein components of exosomes were analyzed by liquid chromatography-mass spectrometry.Through online database analysis,the function of exosome protein was predicted.Results A total of 951 proteins were identified by liquid chromatography-mass spectrometry.The proteins identified by GO functional clustering analysis were related to 28 biological processes,19 cell compositions,and 19 molecular functions.There were 89 pathways in KEGG pathway analysis.Conclusion Exosomes can be obtained by ultracentrifuge.Proteomic analysis of exosomes is helpful to understand the function of exosomes.It will provide a reference for the clinical application of exosomes.关键词
外泌体/超速离心/液质联用Key words
Exosomes/Ultracentrifuge/Liquid chromatography-mass spectrometry分类
医药卫生引用本文复制引用
胡家,殷爱红..质谱分析小鼠神经母细胞瘤细胞外泌体的蛋白组成[J].医学信息,2024,37(11):79-83,5.基金项目
首都医科大学本科生科研训练项目(编号:2023052) （编号:2023052）
