中国动物检疫2024，Vol.41Issue(5)：59-63,5.DOI:10.3969/j.issn.1005-944X.2024.05.013
牛结节性皮肤病血清学检测方法研究进展
Research Progress on Serological Detection Methods for Lumpy Skin Disease
摘要
Abstract
Lumpy skin disease(LSD)has been posing a threat to China's cattle industry,which could be detected by serological methods that were convenient,sensitive and economic,and early detection and evaluation on vaccination effect were also crucial for disease control.In the paper,research progress on various common serological methods for detection of LSD was introduced,the advantages and disadvantages of each method as well as its applicable scenarios were analyzed.Viral neutralization test(VNT),as the gold standard for detecting antibodies against LSD virus,could not be used in the grassroots laboratories due to its long test period and strict operational requirements,but was more suitable for confirming suspected cases;Enzyme-linked immunosorbent assay(ELISA)was appropriate for detection of large number of samples due to its accessibility and short reaction time,but the immune mechanism of antigens was less developed with immature methods;Indirect immunofluorescent antibody test(IFAT)was difficult for clinical detection due to its low specificity in spite of high sensitivity;Immunoperoxidase monolayer assay(IPMA)could be used to detect certain samples,but the results were subject to single-hole microscopy,which was not as clear as those by ELISA;Western blotting(WB)could identify parapoxviruses,but couldn't be used in detection large quantities of samples due to its cumbersome operation.In a word,ELISA was superior in clinical serological detection of LSD.Basic researches on LSD virus should be improved in the future to clarify the immune mechanism of each protein,and to screen out effective and sensitive antigens,so as to establish a method for detection of large quantities of samples at the grassroots level.关键词
牛结节性皮肤病/山羊痘病毒/绵羊痘病毒/血清学检测Key words
LSD/goat pox virus/sheep pox virus/serological detection分类
农业科技引用本文复制引用
任炜杰,邹艳丽,刘春菊,刘珊,赵永刚,李金明,王志亮..牛结节性皮肤病血清学检测方法研究进展[J].中国动物检疫,2024,41(5):59-63,5.基金项目
中国动物卫生与流行病学中心创新基金项目(DW2022003) （DW2022003）
