中国临床药理学与治疗学2024，Vol.29Issue(6)：621-628,8.DOI:10.12092/j.issn.1009-2501.2024.06.003
蛋白激酶全抑制分析揭示KG-1细胞增殖的分子机制
Comprehensive protein kinase inhibition analysis reveals the molecu-lar mechanism of KG-1 proliferation
摘要
Abstract
AIM:To investigate the molecular mechanisms of KG-1 cell proliferation by profiling its responses to various protein kinase inhibitors.METHODS:CCK-8 assay,real time quantitative PCR(qRT-PCR)and Western-blot were used to detect the effect of various protein kinase inhibitors on KG-1 cell proliferation,the expression levels of mRNA and phosphorylation level of signaling pro-teins in the FGFR1 downstream pathways.RE-SULTS:NVP-BGJ398 and PD173074 effectively in-hibited the proliferation of KG-1 cells,indicative of a crucial role of FGFR downstream signaling.After treatment with FGFR inhibitors,the levels of p-FG-FR1OP2-FGFR1 and p-STAT5 decreased significantly(P<0.001),p-AKT decreased slightly(P<0.05),with-out affecting the p-ERK level(P>0.05).CONCLU-SION:FGFR1OP2-FGFR1 mainly acts on the down-stream STAT5 signaling pathway to promote cell proliferation.Comprehensive protein kinase inhibi-tion analysis is a reliable and direct approach to identify functional drivers of cancer cell prolifera-tion.关键词
KG-1细胞/蛋白激酶抑制剂/FGFR1OP2-FGFR1融合基因/STAT5Key words
KG-1 cell line/protein kinase inhibi-tors/FGFR1OP2-FGFR1fusion gene/STAT5分类
医药卫生引用本文复制引用
段毓,徐凝馨,曹琼,杨恺,王金娟,刘思瑾,贾峰峰,刘建兵,李莉..蛋白激酶全抑制分析揭示KG-1细胞增殖的分子机制[J].中国临床药理学与治疗学,2024,29(6):621-628,8.基金项目
山西省留学归国留学生科研资助计划(2020-076) （2020-076）
山西省自然科学基金(自由探索类)(20210302123307) （自由探索类）
国家自然科学基金面上项目(82272622) （82272622）
