中国临床药理学与治疗学2024，Vol.29Issue(6)：629-636,8.DOI:10.12092/j.issn.1009-2501.2024.06.004
抗震止痉方对亚急性帕金森模型小鼠的病程进展干预研究
Intervention study on the progress of subacute Parkinson's disease in mice with Kangzhen Zhijing spasmodic decoction Ⅰ
摘要
Abstract
AIM:To observe the intervention ef-fect of Chinese medicine Kangzhen Zhijing spas-modic decoction Ⅰ on the progression of Parkin-son's disease in mice,and to explore the potential mechanism of neuroprotection.METHODS:Thirty-six C57BL/6 mice were randomly divided into con-trol group,model group,drug group and positive drug group.In the model group,the drug adminis-tration group and the positive drug group,1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)(30 mg/kg)was injected intraperitoneally for 5 days to establish the mouse model.The control group was given the same amount of normal saline,the drug administration group was given 1.25 mg/kg,2.5 mg/kg,5 mg/kg dose of Chinese medicine Kang-zhen Zhijing spasmodic DecoctionⅠby gavage,and the positive drug group was given 75 mg/kg Madopar by gavage.Behavioral tests were per-formed on the second day after the administration in each group.Immunopositive cells of Tyrosine hy-droxylase(TH),Amyloid precursor protein(APP),α-synuclein(α-syn)and solute carrier family 6 mem-ber 11(SLC6A11)were detected by immunohisto-chemistry.The protein expressions of TH,APP,α-syn and SLC6A11 in each group were detected by Western blot.RESULTS:It could significantly im-prove the weight loss of mice in the model group,alleviate the decline of autonomic activity in open field test and the decline of coordination ability in pole test and suspension test.In the reverse immu-nohistochemistry and Western Blot experiments,the expressions of TH and SLC6A11 in the brain of the Parkinson's model group were significantly de-creased,and the expressions of APP and α-syn were significantly increased.CONCLUSION:Kang-zhen Zhijing spasmodic decoctionⅠcan significantly improve the behavioral performance of Parkinson's disease mice and effectively improve the reduction of neuronal transmitters.关键词
动物模型/行为学/帕金森/神经保护/神经退行性疾病Key words
animal model/behavioral science/Parkinson's disease/neuroprotection/neurodegen-erative diseases分类
医药卫生引用本文复制引用
白雪纯,陈硕,李珊珊,李庆林..抗震止痉方对亚急性帕金森模型小鼠的病程进展干预研究[J].中国临床药理学与治疗学,2024,29(6):629-636,8.基金项目
特色皖药(黄精、凤丹、茯苓)综合利用与开发研究(GXXT-2020-025) （黄精、凤丹、茯苓）
2023年安徽省高校自然科学研究项目(2023AH050742) （2023AH050742）
安徽中医药大学自然科学研究项目计划(2021zrzd05) （2021zrzd05）
