中国临床药理学杂志2024，Vol.40Issue(11)：1556-1559,4.DOI:10.13699/j.cnki.1001-6821.2024.11.003
银杏叶提取物调控PI3K/AKT信号通路对人脑血管外膜成纤维细胞增殖和凋亡的影响
Effects of Ginkgo Biloba extract regulating PI3K/AKT signaling pathway on proliferation and apoptosis of human cerebral vascular adventitia fibroblasts
摘要
Abstract
Objective To study the effects of Ginkgo biloba extract(GBE)on the proliferation and apoptosis of human cerebral adventitia fibroblasts(HBVAFs)by regulating the phosphatidylinositol 3 kinase(PI3K)/protein kinase B(AKT)signaling pathway.Methods HBVAFs cells were divided into blank group(normal culture,no treatment),experimental-L,-M,-H groups(25,50 and 100 mg·L-1 GBE treatment for 24 h)and combined group(100 mg·L-1 GBE treatment and 2 nmol·L-1 insulin-like growth factor-1 treatment for 24 h).Cell proliferation was detected by thiazole blue assay,cell apoptosis was detected by flow cytometry,and the protein expressions of phosphorylated-PI3K(p-PI3K),PI3K、phosphorylated-AKT(p-AKT)and AKT were detected by Western blot.Results The cell survival rates of experimental-L,experimental-M,experimental-H,combined and blank groups were(66.78±8.21)％,(53.51±7.40)％,(42.33±6.01)％,(75.69±5.87)％and(96.77±12.80)％;the apoptosis rates were(24.83±2.47)％,(30.07±3.06)％,(37.02±4.66)％,(16.71±2.11)％and(8.99±0.19)％;p-PI3K/PI3K ratios were 0.75±0.07,0.62±0.06,0.43±0.04,0.72±0.05 and 0.90±0.09;p-AKT/AKT ratios were 0.70±0.07,0.60±0.06,0.38±0.03,0.69±0.06 and 0.90±0.09,respectively.The above indexes of the experimental-L,-M,-H groups were compared with the blank group,and the above indexes of the combined group were compared with the experimental-H group,the differences were statistically significant(all P＜0.05).Conclusion GBE can reduce the proliferation of HBVAFs,promote apoptosis,and its mechanism is related to the inhibition of PI3K/AKT signaling pathway.关键词
银杏叶提取物/磷酸肌醇3激酶/蛋白激酶B信号通路/人脑血管外膜成纤维细胞/增殖/凋亡Key words
Ginkgo biloba extract/phosphoinositol 3 kinase/protein kinase B signaling pathway/human cerebral adventitia fibroblasts/proliferation/apoptosis分类
医药卫生引用本文复制引用
毕晓晨,孔晓彬,赵娜,顾晶晶..银杏叶提取物调控PI3K/AKT信号通路对人脑血管外膜成纤维细胞增殖和凋亡的影响[J].中国临床药理学杂志,2024,40(11):1556-1559,4.基金项目
国家自然科学基金青年科学基金资助项目(81603487) （81603487）
