摘要
Abstract
Objective To investigate the effect of tetrandrine on reversing cisplatin resistance in hepatocellular carcinoma cells by up-regulating phosphatase and tensin homolog deleted on chromosome ten(PTEN)expression.Methods Huh7/DDP cells were divided into control group,NC group,group a and group b.The control group received no treatment and was cultured with normal medium only.In NC group,16 μmol·L-1 cisplatin was added into normal medium.In group a,16 μmol·L-1 cisplatin and 5 μmol·L-1 tetrandrine were added into normal medium.In group b,16 μmol·L-1 cisplatin and 5 μmol·L-1 tetrandrine were added to normal medium,and PTEN was overexpressed.Cell survival was assessed by cell counting kit-8(CCK-8)method,and PTEN gene expression was detected by immunofluorescence.The impact on multidrug resistance associated protein(MRP)-3 and MRP-5 were observed.Results The cell survival rates of control group,NC group,a group and b group were(98.74±9.26)％,(55.08±6.38)％,(35.17±4.87)％and(24.06±4.16)％;the expression of PTEN gene were 1.00±0.13,1.03±0.13,1.58±0.17 and 2.18±0.24;the expression of MRP-3 protein were 1.00±0.08,1.03±0.14,0.71±0.07 and 0.48±0.04;MRP-5 protein expression were 1.00±0.11,0.97±0.12,0.63±0.07 and 0.40±0.05,respectively.Compared between NC group and control group,compared between group a and group b,there were statistically significant differences in the above indexes(all P＜0.05).Conclusion Tetrandrine reverses cisplatin resistance in hepatocellular carcinoma cells by up-regulating PTEN expression and inhibits the expression of drug resistance-related proteins MRP-3 and MRP-5.关键词
粉防己碱/肝细胞癌/顺铂耐药性/人第10号染色体缺失的磷酸酶Key words
tetrandrine/hepatocellular carcinoma/cisplatin resistance/phosphate and tension homology deleted on chromosome ten gene分类
医药卫生
