中国临床药理学杂志2024，Vol.40Issue(11)：1598-1602,5.DOI:10.13699/j.cnki.1001-6821.2024.11.012
芹糖葛根素对非酒精性脂肪肝小鼠肝脂代谢的影响
Effects of puerarin apioside on hepatic lipid metabolism in non-alcoholic fatty liver disease mice
摘要
Abstract
Objective To investigate the preventive effects of puerarin apioside on a non-alcoholic fatty liver disease(NAFLD)mouse model and its influence on hepatic lipid metabolism.Methods Thirty-two male C57BL/6J mice were randomly divided into the normal group,model group and experimental-L,-H groups,with 8 mice in each group.The normal group was given regular maintenance feed;the other 3 groups were fed to construct NAFLD model.Experimental-L,-H groups were given 5 and 10 mg·kg-1 puerarin apioside by gavage.Normal and model groups were treated with an equal amount of dimethylsulfoxide by gavage.Four groups were administered once a day for 8 weeks.Changes in mouse body weight were measured.Serum levels of glutamic-pyruvic transaminase(GPT)and glutamic-oxaloacetic transaminase(GOT)and the levels of total cholesterol(TC)and triglycerides(TG)in hepatic tissue were determined by kit.Western blot was used to evaluate the expression levels of acetyl-coenzyme A carboxylase 1(ACC1)and phosphorylated 5'-adenosine monophosphate-activated protein kinase(p-AMPK).Results The body weights of mice in the normal,model and experimental-L,-H groups were(32.69±1.37),(45.51±3.29),(41.18±3.22)and(38.28±2.62)g;serum GPT levels were(36.53±6.44),(134.56±39.91),(121.54±43.38)and(75.92±25.98)U·L-1;serum GOT levels were(60.81±8.74),(188.51±39.70),(156.02±41.67)and(126.79±16.49)U·L-1;hepatic TC levels were(2.17±0.24),(6.46±1.22),(5.86±0.66)and(3.62±0.45)mmol·L-1;hepatic TG levels were(0.57±0.09),(1.39±0.27),(1.28±0.34)and(0.73±0.19)mmol·L-1;the relative expression levels of ACC1 protein were 1.01±0.04,2.00±0.02,1.47±0.08 and 1.20±0.09;the relative expression levels of p-AMPK protein were 1.03±0.09,0.33±0.02,0.66±0.04 and 0.95±0.08,respectively.The differences between the model group and the normal and experimental-H groups were statistically significant for all the above indicators(all P＜0.05).Conclusion Puerarin apioside can improve the hepatic lipid metabolism in NAFLD mice by regulating the AMPK signaling pathway.关键词
芹糖葛根素/非酒精性脂肪肝/脂代谢/5'-磷酸腺苷活化蛋白激酶信号通路Key words
puerarin apioside/non-alcoholic fatty liver disease/lipid metabolism/5'-adenosine monophosphate activated protein kinase signaling pathway分类
医药卫生引用本文复制引用
尚志,胡银璐,徐圣麟,林莉莉..芹糖葛根素对非酒精性脂肪肝小鼠肝脂代谢的影响[J].中国临床药理学杂志,2024,40(11):1598-1602,5.基金项目
国家自然科学基金青年科学基金资助项目(82104606) （82104606）
