中国临床药理学杂志2024，Vol.40Issue(11)：1608-1612,5.DOI:10.13699/j.cnki.1001-6821.2024.11.014
桔梗白散治疗非小细胞肺癌小鼠的作用研究
Effects of Platycodon grandiflorum Bai powder in the treatment non-small cell lung cancer rats
摘要
Abstract
Objective To observe the effects of traditional Chinese medicine compound Platycodon grandiflorum Bai powder on the growth of subcutaneously implanted tumor and the expression of B-cell lymphoma-2(Bcl-2),Bcl-2 associated X protein(Bax),cysteinyl aspartate specific proteinase(caspase)-3 and caspase-9 in subcutaneously implanted tumor of Lewis lung cancer mice.Methods The model of transplanted tumor of Lewis lung cancer in mice was established.Seventy SPF male C57BL/6 mice were randomly divided into blank group,model group,low dose experimental group,medium dose experimental group,high dose experimental group,control group and combined group.Blank group and model group were given 0.9％NaCl 0.2 mL by gavage;control group was given 0.9％NaCl by gavage and 25 mg·kg-1cisplatin intraperitoneally;high,medium,low dose experimental groups were given 193,96,48 mg·kg-1·d-1 Platycodon grandiflorum Bai powder 0.2 mL by gavage,respectively;combined group was given 96 mg·kg-1·d-1 Platycodon grandiflorum Bai powder 0.2 mL by gavage,and 25 mg·kg-1 cisplatin intraperitoneally,once every other day.The myelogenous suppressor cells(MDSCs)of mouse bone marrow were detected by flow cytometry,and the expressions of Bel-2,Bax,caspase-3 and caspase-9 in tumor cells were detected by immunofluorescence.Results The percentage of MDSCs in bone marrow of mice in blank group,model group,low dose experimental,medium,high dose experimental group,control group and combination group were(32.50±2.76)％,(63.13±3.14)％,(48.43±2.23)％,(42.53±1.28)％,(32.93±3.56)％,(51.30±4.25)％and(19.90±6.21)％,respectively.The fluorescence intensities of Bax in model group,low dose experimental group,medium dose experimental group,high dose experimental group,control group and combination group were 10.42±0.68,12.40±1.23,15.14±0.65,22.95±1.76,27.18±1.62 and 31.61±1.28;Bel-2 were 36.85±0.80,33.92±4.20,28.88±1.01,20.04±2.21,15.69±2.36 and 6.05±0.73;caspase-3 were 5.28±0.44,7.63±0.55,9.66±0.85,14.73±1.18,17.95±1.29 and 22.92±1.95;caspase-9 were 9.48±0.90,11.57±0.72,13.45±0.93,15.73±1.44,19.20±0.96 and 23.21±1.51.There were significant differences between medium,high dose experimental groups and model group(all P＜0.05),and there were significant differences between combined group and control group(all P＜0.05).Conclusion Platycodon grandiflorum Bai powder can up-regulating the expression of Bax,caspase-3 and caspase-9,down-regulating the expression of Bel-2,inhibiting MDSCs,promoting tumor cell apoptosis and inhibiting tumor growth.关键词
桔梗白散/顺铂/肺癌/凋亡/B淋巴细胞瘤-2/Bcl-2相关X蛋白/胱天蛋白酶-3Key words
Platycodon grandiflorum Bai powder/cisplatin/lung cancer/apoptosis/B-cell lymphoma-2/Bcl-2 associated X protein/cysteinyl aspartate specific proteinase-3分类
医药卫生引用本文复制引用
袁超,李金田,梁建庆,张毅,孔思琪,胡蓉,张悦,刘瑜..桔梗白散治疗非小细胞肺癌小鼠的作用研究[J].中国临床药理学杂志,2024,40(11):1608-1612,5.基金项目
国家自然科学基金资助项目(82160872) （82160872）
甘肃省教育厅"双一流"科研重点基金资助项目(GSSYLXM-05) （GSSYLXM-05）
甘肃省中医药研究中心开放课题基金资助项目(zyzx-2020-zx17) （zyzx-2020-zx17）
中医学一级学科"岐黄英才"导师专项基金资助项目(2022-13) （2022-13）
甘肃中医药大学研究生创新创业基金资助项目 （）
