中国临床药理学杂志2024，Vol.40Issue(11)：1638-1641,4.DOI:10.13699/j.cnki.1001-6821.2024.11.020
用二维液相色谱法检测人血清中利福喷丁的浓度
Determination of rifapentine concentration in human serum by two-dimensional liquid chromatography
摘要
Abstract
Objective To develop a two-dimensional liquid chromatographic method for rifapentine blood concentration determination.Methods The blood concentration of rifapentine was determined by a novel two-dimensional liquid chromatography(2D-LC)with a one-dimensional column:Aston SC2T(3.5 mm ×50.0 mm,5 μm);a two-dimensional column:Aston SCB(4.6 mm ×250.0 mm,5 μm);the temperature of the column was 40 ℃;the flow rate was 1.0 mL·min-1;the detection wavelength was 335 nm;the injection volume was 300 μL.The specificity,standard curve and lower limit of quantification,precision and recovery,and stability of the method were investigated.The method was used to determine the blood concentration of rifapentine in tuberculosis patients.Results Rifapentine showed good linearity within 0.33-18.62 μg·mL-1 with the standard curve equation of y=2.68 x 105x-5 850.36(r=0.997),the recoveries were 99.81％-105.08％,and the intra-and inter-day precision were ≤4.84％.The results of rifapentine blood concentration measurements in tuberculosis patients were in the range of 0.10-54.70μg·mL-1,and 64.74％were within the therapeutic window concentration range(8-30 μg·mL-1).Conclusion The method is easy to operate,has high sensitivity,low detection limit and high specificity,and is suitable for clinical blood concentration determination.Individual differences in the administration of rifapentine in tuberculosis patients are large,and blood concentration monitoring is required for individualized treatment.关键词
利福喷丁/肺结核/治疗药物监测/二维液相色谱/个体化用药Key words
rifapentine/phthisis/therapeutic drug monitoring/two-dimensional liquid chromatography/individualized medication分类
医药卫生引用本文复制引用
柯涛,何田,张培泽,李巍,周泱,王峰,刘妙娜..用二维液相色谱法检测人血清中利福喷丁的浓度[J].中国临床药理学杂志,2024,40(11):1638-1641,4.基金项目
深圳市科技计划基金资助项目(JC YJ20210324132012035) （JC YJ20210324132012035）
