中国农业科技导报2024，Vol.26Issue(6)：30-44,15.DOI:10.13304/j.nykjdb.2023.0900
缺钾胁迫下谷子转录组分析及相关基因挖掘
Transcriptome Profiling and Gene Mining of Millet Response to Potassium Deficiency Stress
摘要
Abstract
Potassium is an essential element for crop growth.It is of great significance to explore genes that improve crop potassium absorption capacity and enhance crop tolerance to potassium deficiency.In this study,'Jigu 45'was used as material and its seedlings were subjected to potassium deficiency stress for 7 d at the 6-leaf stage.By analyzing agronomic indicators and transcriptome,the relevant genes and expression regulation of potassium deficiency stress were analyzed and explored.The results showed that potassium deficiency stress affected the growth and development of foxtail millet plants:the plant height,leaf width,leaf length,stem width,aboveground dry weight and chlorophyll content were lower then those of CK,while the root length and root surface area significantly increased.Transcriptome analysis showed that 217 genes were upregulated and 38 genes were downregulated after potassium deficiency stress.GO functional enrichment revealed that differentially expressed genes were mainly enriched in the structural components of ribosomes,organic nitrogen compound metabolism processes,protein metabolism processes,cellular protein metabolism processes,and membraneless organelles etc.The KEGG functional enrichment results determined that differentially expressed genes were mainly enriched in 19 pathways,including ribosomes,plant hormone signal transduction and glutathione metabolism etc.Subsequently,35 genes significantly correlated with hormone expression,19 differentially expressed genes related to oxidative stress,10 transcription factor families,and 11 genes related to transduction signals were screened,as well as 1 gene related to sugar metabolism of amino acids and nucleotides.The candidate gene Seita.9G193900 belonged to the CYP45084A subfamily and was a key enzyme regulating S-type lignin synthesis;Seita.5G36500 belonged to WRKY family,which regulated the expression of disease response genes such as PAD3;Seita.3G216900 was an RNA polymerase Ⅱ transcription subunit 37E related mediator,HSPA1s helper protein;Seita.4G286000 was CHB gene which coded chitin endonuclease that regulated sugar synthesis and fermentation.Above results revealed the transcriptional regulation of genes related to potassium deficiency stress in foxtail millet,and laid a foundation for the cloning and functional verification of genes related to potassium tolerance in foxtail millet.关键词
谷子/缺钾/差异表达基因/转录组Key words
millet/potassium deficiency/differentially expressed genes/transcriptome分类
农业科技引用本文复制引用
鲁一薇,夏雪岩,赵宇,崔纪菡,刘猛,黄玫红,褚程,刘建军,李顺国..缺钾胁迫下谷子转录组分析及相关基因挖掘[J].中国农业科技导报,2024,26(6):30-44,15.基金项目
国家谷子高粱产业技术体系建设项目(CARS-06-14.5-A23) （CARS-06-14.5-A23）
河北省农林科学院基本科研业务费包干制项目(HBNKY-BGZ-02) （HBNKY-BGZ-02）
河北省现代农业产业技术体系建设项目(HBCT2024070101,HBCT2024070202). （HBCT2024070101,HBCT2024070202）
